Naslov
Chromium uptake by Saccharomyces cerevisiae and isolation of glucose tolerance factor from yeast biomass

Autori
Gulan Zetić, Vlatka ; Stehlik-Tomas, Vesna ; Grba, Slobodan ; Lutilskky, Lavoslav ; Kozlek, Damir

Izvornik
Journal of Biosciences (0250-5991) 26 (2001), 2; 217-223

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Glucose tolerance factor; Saccharomyces cerevisiae; yeast enriched with chromium

Sažetak
Fermentation with yeast Saccharomyces cerevisiae in semiaerobic and in static conditions with the addition of chromic chloride into the used molasses medium were analysed. The addition of 200 mg/L chrom chloride into the medium stimulated both the yeast growth and the ethanol production in all experimental conditions. On the other hand, the results showed that chrom ions were incorporated into yeast cells during fermentation. Yeast biomass enriched with chromium ions was extracted with 0.1 mol/L NH4OH assuming that the extracts had the glucose tolerance factor (GTF). The presence of GTF in the purified fractions was determined by measuring the absorbanc at 260 nm.it is ecident from the obtained results that the added purified fractions enhanced the rates of CO2 production as well as the glucose utilization during alcoholic fermentation.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA