Naslov
Bioassays - quick proof of Colorado Potato Beetle field resistance

Autori
Lemić, Darija ; Virić Gašparić, Helena ; Čačija, Maja ; Drmić, Zrinka ; Mrganić, Martina ; Bažok, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts ECE 2018 / Dallai, Romano i sur. - Napulj : Event Planet s.r.l, 2018, 224-225

Skup
9th European Congress of Entomology

Mjesto i datum
Napulj, Italija, 02.07.2018. - 06.07.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Potato, Colorado Potato Beetle, insecticide, resistance, LD50, bioassay

Sažetak
The Colorado Potato Beetle (Leptinotarsa decemlineata) (CPB) is one of the most important potato pests worldwide. CPB has a remarkable ability to adjust to toxicants by developing a resistance. More than 300 cases of resistance to 56 insecticides are reported worldwide. Until the mid 1990s CPB resistance was monitored in Croatia and farmers were provided precise data about the status of specific insecticides. Implementation of insecticides with differing modes of action enabled farmers to overcome CPB resistance to organophosphates and pyrethorids. In Croatia recent failures in CPB control indicate that CPB might develop resistance to the newest generation of insecticides. Here we present results from bioassays carried out on CPB larvae with the aim to establish an inexpensive and reliable field resistance monitoring tool. In the bioassays performed, CPB are exposed to different doses of the insecticides and responses (mortality) recorded at the specific post-exposure interval. The mortality data are subjected to Log Dose probit analysis to generate estimates of a lethal dosage that provides mortality to 50% (LD50). When these data are collected for a range of the insecticide-susceptible populations, the LD50 can be used as baseline data for future monitoring purposes. After populations have been exposed to insecticides, the results can be compared to a previously determined LD50 levels using the same methodology. Such bioassays provide a reliable basis for initial characterization of resistant CPB populations, without the need of preserved specimens, expensive equipment or specialized expertise, but require many live specimens and lot of lab work. Despite the bioassays´ drawbacks, they are relatively simple, inexpensive and reliable method to assess resistance of field CPB populations and present a starting point for a more complete understanding of resistance including biochemical and molecular mechanisms that can be undertaken to ultimately build a resistance diagnostic toolbox for this global pest.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA