Naslov
Amyloid precursor protein and neuroplastin – a cause for gender differences in memory impairment upon aging and stress
(Amyloid precursor protein and neuroplastin – a cause for gender differences in memory impairment upon aging and stress)

Autori
Balog, Marta ; Mlinac-Jerković, Kristina ; Ilić, Katarina ; Kalanj-Bognar, Svjetlana ; Zjalić, Milorad ; Ivić, Vedrana ; Gaspar, Robert ; Szucs Kalman ; Vari G, Sandor ; Heffer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
11th FENS Forum of Neuroscience

Mjesto i datum
Berlin, Njemačka, 07.07.2018. - 11.07.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
neurodegeneration ; chronic stress ; lipid rafts

Sažetak
Amyloid precursor protein and neuroplastin – a cause for gender differences in memory impairment upon aging and stress Aims: Validation of gender differences in memory impairment upon aging and stress at the behavioral and molecular level by performing behavioral testing and analyzing membrane distribution of amyloid precursor protein (APP) and neuroplastin in rat hippocampus. Methods: Male and female Sprague Dawley rats were divided in young (6, 5 months old) and old (14, 5 months old) group. Chronic stress and sham stress protocol was performed for 10 weeks. Lipid rafts were isolated from hippocampus. Amyloid precursor protein (APP) and neuroplastin (Np) were screened in lipid raft (LR) and non-lipid raft fractions (NLR) by Western blotting. Memory impairment was tested by passive avoidance test. All data was analyzed by the Statistica software. Results: APP was detected in NLR membrane fractions and Np was detected in both LR and NLR fractions of hippocampus. Np expression upon stress decreased in male and increased in female animal groups. Opposite, APP increased in young males and decreased in young females. Behavioral testing demonstrated memory impairment in old female chronic stress group as compared to young female chronic stress group (U=0, N1=N2=9, p=0.00004) while male animals had no statistically significant changes in memory compared to other animal groups. Conclusions: Chronic stress, not aging itself, causes memory impairment that is specifically observed in old female animals.. Changes in Np and APP upon stress in all animal groups implicate its strong connection with HPA axis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA