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Pregled bibliografske jedinice broj: 946663

Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde

Česnik, Morana; Sudar, Martina; Roldan, Raquel; Hernandez, Karel; Clapés, Pere; Vasić-Rački, Đurđa; Findrik Blažević, Zvjezdana
Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde // European congress of Biotechnology 2018 / Lescai, Francesco ; Rudelsheim, Patric (ur.).
Ženeva, Švicarska: Elsevier, 2018. str. 137-137 doi:10.1016/j.nbt.2018.05.1082 (poster, međunarodna recenzija, sažetak, znanstveni)

CROSBI ID: 946663 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde

Autori
Česnik, Morana ; Sudar, Martina ; Roldan, Raquel ; Hernandez, Karel ; Clapés, Pere ; Vasić-Rački, Đurđa ; Findrik Blažević, Zvjezdana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European congress of Biotechnology 2018 / Lescai, Francesco ; Rudelsheim, Patric - : Elsevier, 2018, 137-137

Skup
European Congress on Biotechnology

Mjesto i datum
Ženeva, Švicarska, 01.07.2018. - 04.07.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aldolna adicija, aldolaza, matematičko modeliranje
(aldol addition, aldolase, mathematical modelling)

Sažetak
Carbon-carbon bond formation between propanal and formaldehyde in aldol addition catalysed by newly developed D-fructose-6-phosphate aldolase variant was investigated in this work. The reaction product is 3-hydroxy-2-methylpropanal (Roche aldehyde) widely used as intermediate in synthetic chemistry. The reaction kinetics was determined and the mathematical model was developed for process optimization. Optimization goals included finding the best conditions and the best reactor mode to obtain maximum product concentration and yield. It was found that the kinetics of the aldol addition can be described by double substrate Michaelis-Menten kinetics with included substrate inhibition by formaldehyde and propanal and noncompetitive inhibition by methanol used as formaldehyde stabilizer. Self-addition of propanal was described by double substrate Michaelis-Menten kinetics and included in the model. It was found that enzyme operational stability decay is co-dependent on the initial formaldehyde concentration. Thus, the choice of the initial conditions is crucial for the successful process set-up. Developed mathematical model described the experimental data very well. At the calculated optimal process conditions in fed-batch as the best reactor choice, the product concentration after 5.5 hours was 814 mmol dm-3 (72 g L–1), product yield was 88.5% and volume productivity was 313.7 g L–1 d–1.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija



POVEZANOST RADA

Projekti:
EK-H2020-635595 - Sustainable industrial processes based on a C-C bond-forming enzyme platform (CARBAZYMES) (Findrik Blažević, Zvjezdana, EK - H2020-LEIT-BIO-2014-1) ( CroRIS)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url MORANA ČESNIK KATULIĆ (autor)

Avatar Url Martina Sudar (autor)

Avatar Url Zvjezdana Findrik Blažević (autor)

Avatar Url Đurđa Vasić-Rački (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Česnik, Morana; Sudar, Martina; Roldan, Raquel; Hernandez, Karel; Clapés, Pere; Vasić-Rački, Đurđa; Findrik Blažević, Zvjezdana
Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde // European congress of Biotechnology 2018 / Lescai, Francesco ; Rudelsheim, Patric (ur.).
Ženeva, Švicarska: Elsevier, 2018. str. 137-137 doi:10.1016/j.nbt.2018.05.1082 (poster, međunarodna recenzija, sažetak, znanstveni)
Česnik, M., Sudar, M., Roldan, R., Hernandez, K., Clapés, P., Vasić-Rački, Đ. & Findrik Blažević, Z. (2018) Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde. U: Lescai, F. & Rudelsheim, P. (ur.)European congress of Biotechnology 2018 doi:10.1016/j.nbt.2018.05.1082.
@article{article, author = {\v{C}esnik, Morana and Sudar, Martina and Roldan, Raquel and Hernandez, Karel and Clap\'{e}s, Pere and Vasi\'{c}-Ra\v{c}ki, \DJur\dja and Findrik Bla\v{z}evi\'{c}, Zvjezdana}, year = {2018}, pages = {137-137}, DOI = {10.1016/j.nbt.2018.05.1082}, keywords = {aldolna adicija, aldolaza, matemati\v{c}ko modeliranje}, doi = {10.1016/j.nbt.2018.05.1082}, title = {Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde}, keyword = {aldolna adicija, aldolaza, matemati\v{c}ko modeliranje}, publisher = {Elsevier}, publisherplace = {\v{Z}eneva, \v{S}vicarska} }
@article{article, author = {\v{C}esnik, Morana and Sudar, Martina and Roldan, Raquel and Hernandez, Karel and Clap\'{e}s, Pere and Vasi\'{c}-Ra\v{c}ki, \DJur\dja and Findrik Bla\v{z}evi\'{c}, Zvjezdana}, year = {2018}, pages = {137-137}, DOI = {10.1016/j.nbt.2018.05.1082}, keywords = {aldol addition, aldolase, mathematical modelling}, doi = {10.1016/j.nbt.2018.05.1082}, title = {Mathematical model based optimization of enzymatic aldol addition of propanal to formaldehyde}, keyword = {aldol addition, aldolase, mathematical modelling}, publisher = {Elsevier}, publisherplace = {\v{Z}eneva, \v{S}vicarska} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • Social Science Citation Index (SSCI)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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