Naslov
RAPD fingerprinting of indigenous Sinorhizobium meliloti strains

Autori
Sikora, Sanja ; Redžepović, Sulejman ; Bradić, Mihaela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Fourth European Nitrogen Fixation Conference, Sevilla, Spain. Congresos y Jornadas 56/00 / Olivares, J. ; Palomares, A.J. - Sevilla : Junta De Andalucia. Consejeria de Agricultura y Pesca, 2000, 335-335

Skup
Fourth European Nitrogen Fixation Conference

Mjesto i datum
Sevilla, Španjolska, 16.09.2000. - 20.09.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
RAPD; Sinorhizobium meliloti; indigenous strains

Sažetak
Effective alfalfa production would have to include application of inoculation with highly efficient Sinorhizobium meliloti strains because of significant reduction in mineral nitrogen fertilization which has important economical and ecological effects. In Croatia, the use of rhizobial inoculants for alfalfa and other forage crop legumes, still hasn`t become the usual procedure although these crops are of great agricultural importance. In order to select the most suitable S. meliloti strains for alfalfa inoculants, we have been started with the isolation of indigenous S. meliloti strains from different field sites in Croatia.The aim of present investigation was to identify different S. meliloti strains and to assess genetic diversity among field isolates obtained from different ecological regions. All field isolates as well as reference strains were analysed by using RAPD fingerprinting. Total genomic DNAs from all strains tested were amplified using four different 10-mer primers. Amplification reactions with arbitrary primers, generated highly specific and reproducibile patterns which enabled strain differentiation. RAPD profiles revealed that all field isolates considerably differed from reference strains. Cluster analysis showed high degree of diversity among field isolates. None of the strains were identical or nearly identical to each other. Among indigenous S.meliloti strains two highly divergent groups can be determined. Although it is well known that indigenous strains are very competitive and usually well adapted to specific ecological conditions, further investigations are needed to evaluate their symbiotic efficiency in order to select strains for high-quality inoculant production.

Izvorni jezik
Engleski

Znanstvena područja
Ekonomija

POVEZANOST RADA