Naslov
Lactic acid production from wheat straw cellulose obtained after alkali pre-treatment in high-pressure reactor by Lactobacillus amylovorus DSM 20531T

Autori
Trontel, Antonija ; Vidović, Petra ; Novak, Mario ; Marđetko, Nenad ; Šantek, Božidar

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Natural resources, green technology & sustainable development - Book of Abstract / Radojčić Redovniković I. ; Jakovljević T. ; Petravić Tominac V. ; Panić M. ; Stojaković R. ; Erdec D. ; Radošević K. ; Gaurina Srček V. ; Cvjetko Bubalo M. - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 2018, 129-129

ISBN
978-953-6893-11-9

Skup
3rd Natural resources green technology & sustainable development-GREEN/3

Mjesto i datum
Zagreb, Hrvatska, 05.06.2018. - 08.06.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
alkali pre-treatment in high-pressure reactor, enzymatic hydrolysis and fermentation, lactic acid, Lactobacillus amylovorus, wheat straw

Sažetak
Sugar and starch containing raw materials are widely used as food and feed thus, second generation raw materials like waste lignocellulosic biomass present sustainable alternative for production of different biochemicals, e.g. lactic acid. In this work, alkali pre-treatment of wheat straw with 2 % w/w sodium hydroxide solution in high-pressure reactor at different temperatures (from 120°C to 210°C) and residence times (from 1 to 20 minutes) was performed. After alkali pre-treatment of wheat straw, solid and liquid phase was obtained and composition of the two phases was determined. Solid phase (wheat straw cellulose) with high glucan content was used to study the fermentation capabilities of selected strain in two bioprocess types: Separate Hydrolysis and Fermentation (SHF) and Simultaneous Cellulose Hydrolysis and Fermentation (SCHF). Cellulose hydrolysis was performed with commercially obtained cellulases and β-glucosidases isolated from mold Aspergillus niger. In SHF bioprocess, after enzymatic hydrolysis of wheat straw cellulose, obtained hydrolysates were used for lactic acid production by lactic acid bacterium Lactobacillus amylovorus DSM 20531T. While, in SCHF bioprocess, enzymatic hydrolysis of wheat straw cellulose to simpler carbohydrates and fermentation by L. amylovorus DSM 20531T was performed simultaneously. Process efficiency parameters, like final lactic acid concentration, lactic acid yield and bioprocess productivity, in SCHF bioprocess were approximately 60 % higher than in SHF bioprocess.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA