Naslov
A simple and reliable new microchip electrophoresis method for fast measurements of imidazole dipeptides in meat from different animal species

Autori
Jozanović, Marija ; Sakač, Nikola ; Sak-Bosnar, Milan ; Carrilho, Emanuel

Izvornik
Analytical and bioanalytical chemistry (1618-2642) 410 (2018), 18; 4359-4369

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
C4D detector ; Electrochemical detection ; Imidazole dipeptides ; Meat samples ; Microfluidics ; Microchip electrophoresis
(C4D detector ; electrochemical detection ; imidazole dipeptides ; meat samples ; microfluidics ; microchip electrophoresis)

Sažetak
Microchip electrophoresis (ME) was applied for the separation of two physiologically important imidazole dipeptides—carnosine and anserine. The capacitively coupled contactless conductivity detector (C4D) was employed for quantification of both dipeptides after separation in a new home-built ME unit. The separation parameters were optimized as follows to enable quantitative, baseline separation of both dipeptides: injection time 16 s, injection voltage 900 V/cm, and separation voltage 377.1 V/cm. The C4D detector responded linearly to both imidazole dipeptides in the range 0–20 mg L−1. The known addition methodology was applied to test the accuracy of the measurement of imidazole dipeptides in a complex sample. The recoveries for measurement of carnosine in the mixture ranged from 96.1 to 105.0%, whereas those for anserine amounted to 96.6 to 102.0%. This method was also applied to real biological samples. The results exhibited a satisfactory agreement with a standard HPLC method. The proposed ME method represents a cheap, fast, and simple alternative to the existing, more complicated and expensive HPLC methods. This method does not demand either the optical detectors nor tedious derivatization of sample, which are unavoidable in HPLC methods. The method was succesfuly applied for animal species determination in unknown meat samples using the carnosine/anserine ratio, and subsequently, it could be used in a food fraud prevention process.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Prehrambena tehnologija, Nutricionizam

POVEZANOST RADA