Naslov
Consequences of long term changes in CHV1 prevalence on natural biological control

Autori
Ježić, Marin ; Mlinarec, Jelena ; Nuskern, Lucija ; Pavičić, Ivana ; Poljak, Igor ; Idžojtić, Marilena ; Schwarz, Janine Melanie ; Prospero, Simone ; Rigling, Daniel ; Ćurković-Perica, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Viruses Book of Abstracts / Bielen, Ana ; Ježić, Marin ; Jurak, Igor ; Škorić, Dijana ; Tomaić, Tomislav - Zagreb : Hrvatsko mikrobiološko društvo, 2018, 84-84

ISBN
978-953-7778-15-6

Skup
The conference Power of Viruses

Mjesto i datum
Poreč, Hrvatska, 16.05.2018. - 18.05.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chestnut blight, Cryphonectria parasitca, hypovirulence, vc diversity

Sažetak
Mycoviruses, mostly dsRNA or +ssRNA subcellular pathogens of fungi, often cause asymptomatic, latent infections of their hosts. Some of them, however, have debilitating effect, reducing fitness and/or reproductive capability of the infected fungus. This might be effective in biological control of pathogenic fungi, such as Cryphonectria parasitica, a phytopathogenic Ascomycota native to eastern Asia which causes the chestnut blight disease. Cryphonectria hypovirus 1 (CHV1) is a mycovirus which lacks an extracellular phase and reduces the fitness of its host, as well as its aggressiveness against the chestnut post CHV1 infection, a phenomenon called hypovirulence. It is transmitted vertically via asexual spores and horizontally over the anastomoses between genetically compatible mycelia (heterokaryonic or vegetative compatibility). This is important because the only way to successfully control chestnut blight disease is via biological control of the fungus, utilizing CHV1. Approximately a decade ago, CHV1 prevalence in Croatia was determined to be between 13% (in coastal regions) and up to about 60% in some continental populations. Recently, we have revisited three populations and surveyed them for CHV1 prevalence and genetic diversity (vegetative compatibility – vc) of the fungus, which is a major determinant of the successful horizontal spread of the CHV1 and biological control of the disease. CHV1 prevalence increased in coastal population Buje from 12, 7% to 19, 5% and decreased in continental populations: from 50, 8% to 29, 9% in Hrvatska Kostajnica and from 44, 1% to 33, 3% in Ozalj. At the same time vc type diversity increased in all populations: previously between five and eight vc types were observed in the three mentioned populations, while recently as many as 19 different vc types have been recorded, Shannon diversity index reflecting that increase. In our continued effort to monitor the disease development with shorter sampling intervals, in the last three years we have revisited the same locations and observed somewhat quicker pace of canker area increase in population with lower CVH1 prevalence (Buje) than in populations with higher CHV1 prevalence. This obviously has a major impact on the success of biological control, since CHV1 seems to decrease in prevalence in both continental populations. All this might indicate collapse of the established biological control in those populations. The reason behind this is the increase in genetic diversity of the C. parasitica which negatively affects successful horizontal transfer of the virus.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo

POVEZANOST RADA