Naslov
DNA methylation profiling in head and neck cancer

Autori
Milutin Gašperov, Nina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Programme and Abstract Book of the International Symposium on Epigenetic Changes in Head and Neck Cancer / Grce, Magdalena - Zagreb, 2018, 10-10

Skup
International Symposium on Epigenetic Changes in Head and Neck Cancer

Mjesto i datum
Zagreb, Hrvatska, 27.04.2018

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Epigenetic ; DNA methylation ; Head and Neck Cancer

Sažetak
It is well established that the main risk factors associated with Head and Neck Squamous Cell Carcinoma (HNSCC) are excessive tobacco and alcohol consumption together with high-risk types of human papillomavirus (HPV). Still, there is a strong need to find new, good biomarkers of this disease. The most appropriate biomarkers would be those that would point out changes in epithelial cells before carcinoma occurrence. DNA methylation is an irreversible change that inhibits gene expression. Thus, altered DNA methylation is one of the possible factors associated with the HNSCC development. The presumption is that epigenetic biomarkers, such as methylated genes could point to changes even before they can be clinically detected. The model on which we explore those changes of DNA methylation are normal oral mucosa tissue, potentially malignant lesions of the oral mucosa, and HNSCC tissue. Oral precancerous lesions are difficult to distinguish clinically and histopathologically, so early detection by epigenetic tools would be important in further patent treatment. Herein, we will discuss DNA methylation profiling obtained by Infinium MethylationEPIC BeadChip array, pyrosequencing and methylation specific polymerase chain reaction (MSP) and their correlation with the diagnosis. One of the goal of the on-going Epic-HNSCC research project was to reveal DNA methylation differences in normal oral samples and HNSCC samples. The results obtained with Infinium MethylationEPIC BeadChip array showed a panel of gene promoters significantly methylated in HNSSC samples in comparison to normal samples, mainly of genes related with receptor and transmembrane functions and transcription activity. The group of hypomethylated gene promoters in HNSCC, in comparison to normal oral mucosa, was mainly involved in immune responses.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA