Naslov
Role of ST3GAL5 polymorphism (rs72842068) in cervical cancer development

Autori
Danolic, Damir ; Heffer, Marija ; Wagner, Jasenka ; Škrlec, Ivana ; Alvir, Ilija ; Mamic, Ivica ; Susnjar, Lucija ; Banovic, Marija ; Puljiz, Mario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Journal of Gynecological Cancer / - , 2017, 728-728

Skup
20th International Meeting of the European Society of Gynaecological Oncology

Mjesto i datum
Beč, Austrija, 04.11.2017. - 07.11.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cervical cancer ; polymorphism ; ST3GAL5

Sažetak
Aims: To determine whether polymorphisms in the ST3GAL5 gene represents risk factor for cervical cancer. Method: In total, 164 women participated in our study. Peripheral blood samples were obtained from 81 cervical cancer patients and 83 healthy controls at University Hospital for Tumors, Zagreb, Croatia. The diagnosis of cervical cancer was confirmed by histological examinations of tissues from biopsies or resected specimens. The controls were represented by age-matched females with a normal Pap smear and no previous history of cervical dysplasia or malignant disease. Informed written consent was obtained from all participants. A 3 mL sample of venous blood was collected from each subject into a test tube containing EDTA as anticoagulant. Genomic DNA was extracted by QIAamp DNA Blood Mini Kit (Qiagen Company) according to the manufacturer’s protocol. The ST3GAL5 (rs72842068) single nucleotide polymorphism was genotyped with TaqMan allele- specific PCR amplification technology on an Applied Biosystems 7500/7500 Real-Time PCR System. Results: There was no significant difference between the two groups in the frequency distributions of age (P = 0.786) suggesting that matching of subjects based on this variable was adequate. We detected only one genotype for the ST3GAL5 (rs72842068) polymorphism. All genotyped subjects carried the homozygous wild-type genotype AA, while heterozygous genotype AG and homozygous genotype GG were not detected. Conclusion: The present study did not show association of the ST3GAL5 polymorphism (rs72842068) with cervical cancer, but it opened future research direction. In our opinion, the relationship between ganglioside biosynthesis and cervical cancer is far from being elucidated.

Izvorni jezik
Engleski

Znanstvena područja
Grafička tehnologija, Glazbena umjetnost

POVEZANOST RADA