Naslov
A host miRNA cluster miR-183/96/182 is upregulated during herpes simplex virus 1 productive infection

Autori
Badurina, Maja ; Zubković, Andreja ; Ratkaj, Ivana ; Jurak, Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the 42nd International Herpesvirus Workshop / - , 2017, 159-159

Skup
42nd International Herpesvirus Workshop

Mjesto i datum
Gent, Belgija, 29.07.2017. - 02.08.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Herpes simplex virus 1, host microRNAs

Sažetak
Many viruses deregulate expression of host miRNAs to facilitate their replication or establish and maintain latency. Herpes simplex virus 1 (HSV-1) encodes a large number of miRNAs and it has been shown to impact host miRNA expression during productive infection. However, these studies were largely limited to the analyses on only one cell line in which single miRNAs were detected up- or down-regulated. To comprehensively investigate changes in host miRNA expression during productive infection, we first sequenced small-RNAs from the productively infected HFFs at different times after infection. We observed a change in expression levels for a number of miRNAs, however levels of only a few were changed for more than 3x ; and among these were three miRNAs expressed from a single cluster, miR-183/96/182. Interestingly, these miRNAs were previously found upregulated in HSV-1 infected primary neurons (A. Wilson and D. Coen personal communication). Next we analysed the expression levels of eight cellular miRNAs, most of which have been previously reported deregulated in HSV-1 infection or were found in our screen, in three cell lines (human embryonic kidney cells HEK293, neuroblastoma cells SH-SY5Y, human fibroblasts WI38) and two primary cells (human foreskin fibroblasts (HFFs) and mouse bone marrow derived macrophages (BMDM) at different time points after infection. Surprisingly, we did not detect a significant change for any of the analysed miRNAs in any cell type but for miR-182, miR-96 and miR-183 in fibroblasts (WI38 and HFFs ; ~10x). Our preliminary results show that the upregulated cellular miRNAs, as well as HSV-1 expressed miRNAs, are loaded successfully onto RISC complex, which might indicate their function. Indeed, we observed decreased protein levels for several predicted targets of miR-182, miR-96 and miR-183, which coincides with the increased expression of these miRNA. Nonetheless, thus far, our results show that most of the observed phenomena were not related to the miRNA upregulation, but rather to other virus functions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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