Pregled bibliografske jedinice broj: 934035
Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers
Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers // Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, 12 (2017), 3-4; 113-119 (međunarodna recenzija, članak, znanstveni)
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Naslov
Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers
Autori
Mario, Novak ; Pavlečić, Mladen ; Harutyunyan, Baghish ; Goginyan, Vigen ; Horvat, Predrag ; Šantek, Božidar
Izvornik
Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam (1847-3423) 12
(2017), 3-4;
113-119
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
purple non-sulphur bacteria ; Rhodobacter capsulatus ; 5-aminolevulinic acid ; optimization of cultivation conditions.
Sažetak
Wild strains of purple non-sulphur bacteria: Rhodospirillum rubrum B-6505, Rhodopseudomonas palustris B-6506, Rhodobacter capsulatus B-6508 and Rhodobacter spheroides B-6509 were studied as 5-ALA (5 aminolevulinic acid) producers. Selected strains were subjected to mutagenesis with N-methyl-N-nitro-N-nitrosoguanidine to obtain a strain with high 5-ALA producing capacity. After mutagenesis 19 stable mutant strains were selected from Rhodobacter capsulatus B-6508 and Rhodobacter sphaeroides B-6509. On the basis of obtained results, mutant strain of Rhodobacter capsulatus B-6508 has shown the highest potential for 5-ALA production. The most favorable conditions for growth and 5-ALA production by mutant strain R. capsulatus B-6508 were observed in media composed of glutamate and malate, light at 2000 Lux, microaerophilic conditions and temperature of 28 °C. In these conditions, the highest 5- ALA concentration (179 mg/L) was detected together with the highest bacterial physiological activity. The prolongation of mutant strain R. capsulatus B-6508 cultivation time after glycine, succinate and levulinic acid addition is related to the reduction of 5- ALA concentrations (e.g. 124.5 mg/L after 48 h and 89.5 mg/L after 72 hours). In the light/aerobic conditions R. capsulatus B-6508 produced only 58.1 mg/L of 5 ALA. Furthermore, in dark conditions even lower biomass and 5-ALA concentrations were observed during R. capsulatus B-6508 cultivation.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
POVEZANOST RADA
Projekti:
EK-H2020-690925 - People for tHe eurOpean bioENergy mIX (Phoenix) (Šantek, Božidar; Krajačić, Goran, EK - H2020-MSCA-RISE-2015) ( CroRIS)
KK.01.1.1.01.0002 - Bioprospecting Jadranskog mora (Jerković, Igor; Dragović-Uzelac, Verica; Šantek, Božidar; Čož-Rakovac, Rozelinda; Kraljević Pavelić, Sandra; Jokić, Stela, EK ) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
