Naslov
DETECTION OF VIRULENCE GENE BELONGING TO CAG PATHOGENICITY ISLAND IN HELICOBACTER PYLORI ISOLATES AFTER MULTIPLE UNSUCCESSFUL ERADICATION THERAPY IN NORTHWEST CROATIA

Autori
Varda-Brkić, Dijana ; Katičić, Miroslava ; Bedenić, Branka ; Presečki-Stanko, Aleksandra ; Plečko, Vanda

Izvornik
Periodicum biologorum (0031-5362) 118 (2016), 1; 45-52

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Helicobacter pylori, genotyping, cag pathogenicity island, cagA

Sažetak
Background: Some of the genes belonging to cag pathogenicity island (cagPAI) in Helicobacter pylori were found to be associated with an increased severity of gastric mucosal inflammation that might lead to the development of gastroduodenal disease. The most characterized is cytotoxin associated gene A (cagA) encoding cagA protein with proven onocogenic properties. Aim: The aim of our study was to define a group of patients with necessary eradication therapy based on the detection of virulence genes of cagPAI in H. pylori and comparison with pathohistological finding. Methods:In total 103 H. pylori isolates were analysed. Genes encoding virulence factors were detected by PCR with primers for 10 loci in cagPAI: Apcag (cagA promotor region), cagA1, cagA2, cagA3, cagM, cagE, LEC, tnpA, and tnpB. The patients who provided isolates were classified into three clinical categories: Un ulcer dyspepsia (n=69), ulcus ventriculi (n=22) and ulcus duodeni (n=12). Results: 16 strains (15.5%) were negative for all tested genes. 87 (84.5%) of the isolates had parcially deleated cagPAI. None of the isolates possessed all 10 genes. The frequency of single cagPAI genes were as follows: Apcag 63.1%, cagA1 71.8%, cagA2 69.9%, cagA3 5.8%, cagM 71.8%, cagE 68%. tnpA 9.7%, tnpB 7.8% i LEC 48.5%. No statistically significant difference was observed between the presence of any cagPAI genes and endosopic diagnosis (p>0.16). The presence of CagA2, Apcag and cagM showed statistically significant correlation with higher level of histological parameters of gastritis (p<0.05). Conclusions: H. Pylori isolates with positive cagA, Apcag, and cagM genes are correlated to higher degree of patohistological lesions of gastric mucosa ; without statistically significant correlation with endoscopic diagnosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA