Naslov
Mapping quantitative trait loci for Ascochyta fabae Speg. resistance in faba bean (Vicia faba L.)

Autori
Roman, Belen ; Avila, Carmen Maria ; Torres, Ana Maria ; Rubiales, Diego ; Kolak, Ivan ; Moreno, Maria Teresa ; Šatović, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th Quantitative Trait Locus Mapping and Marker-Assisted Selection Workshop / Carbonell, Emilio ; Blasco, Agustin - Valencia : Universitat Politècnica de València (UPV), 2001, 37-38

Skup
7th Quantitative Trait Locus Mapping and Marker-Assisted Selection Workshop

Mjesto i datum
Valence, Francuska, 19.10.2001. - 20.10.2001

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
faba bean; Vicia faba L.; Ascochyta fabae Speg.; QTL; resistance; SIM; CIM

Sažetak
Ascochyta blight, incited by Ascochyta fabae Speg., is widely distributed faba bean (Vicia faba L.) disease. Although resistant sources have been identified, little is known on its genetic basis and gene location. RAPD and isozyme analyses were carried out using plant tissue from 196 individual F2 plants derived from the cross between susceptible and resistant line. F2-derived F3 lines were inoculated in growth chamber conditions and evaluated for disease severity considering the percentage of damaged leaf area. The linkage map was constructed by MAPMAKER V2.0 (Lander et al., 1987), while the QTL analysis was carried out using QTL Cartographer (Basten et al., 2001). One-hundred and twenty-one out of 130 marker loci could be mapped into 16 linkage groups. Nine linkage groups were assigned to their specific chromosomes because a number of markers was common with previous studies (Torres et al., 1993; Satovic et al., 1996; Vaz Patto et al., 1999) that used primary trisomics in order to establish the chromosomal location of markers / genes. By Simple Interval Mapping (SIM) two zones of putative QTL action were detected. Using Forward-Backward Stepwise Regression four markers were selected to be used as cofactors in Composite Interval Mapping (CIM). Putative QTLs (Afl1, and Afl2) were identified on linkage groups VIII (chromosome 3), and IVa (chromosome 2), respectively, jointly contributing about 45 % of the total phenotypic variance. Afl1 displayed pure additive gene action while Afl2 had considerable dominant effect.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

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