Naslov
Identification of key molecules underlying spinal cord regeneration using South-American opossum (Monodelphis domestica)

Autori
Mladinić Pejatović, Miranda ; Tomljanović, Ivana ; Petrović, Antonela ; Ban, Jelena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 6. Croatian Neuroscience Congress with international participation / - , 2017, 52-52

Skup
6. Croatian Neuroscience Congress with international participation

Mjesto i datum
Osijek, Hrvatska, 16.09.2017. - 18.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
mammalian central nervous system, marsupials, neuroregeneration, stem cells

Sažetak
One of the major challenges of modern biology concerns the inability of the adult mammalian central nervous system (CNS) to regenerate and repair itself after injury. Unlike the situation in adult mammals, lower vertebrates, such as fish and amphibians, and embryonal higher vertebrates can regenerate significant portion of their CNS. It is poorly understood why this potential is lost with evolution and development and why it becomes very limited in adult mammals. A preferred model to study and reveal the cellular and molecular basis of this loss is neonatal opossum (Monodelphis domestica). Opossums are marsupials that are born at very immature stage with unique possibility to successfully regenerate spinal cord after injury in the first two weeks of their life. They offer an exceptional opportunity to study the mammalian CNS that still can regenerate, without a need of invasive intrauterine surgery of pregnant females (like necessary for other mammalian laboratory animals, such as mouse or rat. In the previous studies we have identified the genes that changes their expression at the time when regeneration in opossum stops being possible. Now, we are analyzing the proteoms of the opossums of different age (that can and cannot regenerate spinal cord tissue after injury), looking for the new molecules associated with regenerative axonal growth. The activity of candidate molecules will be inhibited to test their functional role in neuronal regeneration using in vitro intact spinal cord preparation and advanced imaging. In parallel, wa are preparing and analyzing the opossum primary spinal cultures made from animals of different age, to test candidate molecules, but also to reveal dissimilarities in the cellular content (giving focus on stem cells) and metabolic characteristics related to regeneration. The results of the project could make substantial contribution to our understanding of neuronal regeneration in mammals, but also provide canididate targets for future novel therapeutic interventions for neurodegenerative disorders.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA