Naslov
Stereoselective diol production by multi-enzyme system

Autori
Vrsalović Presečki, Ana ; Pintarić, Lela ; Švarc, Anera ; Vasić-Rački, Đurđa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract of European Biotechnology Congress 2017 held in Dubrovnik, Croatia during 25 - 27 May 2017 / Dundar, Munis - Amsterdam : Elsevier, 2017, S10-S10

Skup
European Biotechnology Congress 2017

Mjesto i datum
Dubrovnik, Hrvatska, 25.05.2017. - 27.05.2017

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
multi-enzyme cascade reaction, asymmetric reduction, stereoselectivity, alcohol dehydrogenase, benzoylformate decarboxylase

Sažetak
Within the field of biocatalysis, multi-enzyme cascade reactions are an important strategy for the production of optically active chemicals. This approach is very promising from an environmental and as well the economical point of view since in this manner higher yields and productivities is accomplished, fewer chemicals are spent, isolation of intermediates is avoided and on industrial scale, waste and production costs are reduced. The synthesis of chiral building blocks is always challenging in the field of organic chemistry, especially the production of molecules with two chiral centers, as the chiral 1, 2-diols. These have wide application as synthons for chemical catalysts, agrochemicals and pharmaceuticals and can be produced by chemical and enzymatic methods. The production of specific form of the stereoisomer of 1, 2-diols by enzymatic approach seems to be more attractive since the synthesis takes place under mild conditions and result with high stereoselectivity. An example of multienzyme catalysis for the production of chiral 1, 2 diol will be presented. By coupling stereoselective carboligation reaction catalyzed by benzoylformate decarboxylase, L- selective reduction of carbonyl group with alcohol dehydrogenase and the coenzyme regeneration by formate dehydrogenase, enantiometrically pure diastereoselective diol was successfully produced from inexpensive substrates.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA