Naslov
Application of innovative and rapid flow cytometry method for determination of psychrotrophic bacteria in raw milk

Autori
Krga, Mateja ; Zamberlin, Šimun ; Špoljarić, Jasminka ; Radeljević, Biljana ; Horvat Kesić, Iva ; Pejić, Martina ; Plavljanić, Dijana ; Antunac, Neven ; Mikulec, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
53rd Croatian and 13th International Symposium on Agriculture Book of Abstracts / Rozman, Vlatka ; Antunović, Zvonko - Osijek : Fakultet agrobiotehničkih znanosti Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2018, 211-213

Skup
53. hrvatski i 13. međunarodni simpozij agronoma

Mjesto i datum
Vodice, Hrvatska, 18.02.2018. - 23.02.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
psychrotrophic bacteria, flow cytometry, IBC, CFU, conversion line

Sažetak
Cooling and longer storage of raw milk at low temperatures favors the growth of psychrotrophic bacteria which pose a serious problem for the dairy industry, as they affect the deterioration, sustainability and milk yield during processing. Therefore, the number of these bacteria in cooled milk compared to the number of mesophilic aerobic bacteria is significantly higher than their ideal ratio of 10%. For the industry it is of crucial importance to rapidly determine the number of psychrotrophic bacteria in raw milk. Flow cytometry method compared to the time consuming reference method is significantly different in the expression of the results. Flow cytometry method determines the total individual bacterial count (IBC) that represents the total number of living and non-living bacteria and needs to be converted to the total number of living psychrotrophic bacteria (CFU). The aim of this study was to establish the regression equation for psychrotrophic bacteria by which the IBC is converted into CFU. In 287 raw milk samples the IBC was determined by flow cytometry method and the CFU by reference method (ISO 6730). The conversion line y= 1, 0594x-0, 7874 was calculated by using linear regression (ISO 21187) where y represents the logarithmic value of CFU and x the logarithmic value of the IBC. The established conversion line will enable the dairy industry to quickly and reliably determine the total number of psychrotrophic bacteria in raw milk.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA