Naslov
Translational research in Croatia – combining clinical knowledge and advanced technologies for studying IBD

Autori
Panek Marina ; Perić Mihaela ; Barešić Anja ; Čipčić Paljetak Hana ; Matijašić Mario ; Kunović Ana ; Vranešić-Bender Darija ; Urek Marija ; Krznarić Željko ; Verbanac Donatella

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Found in Translation Conference

Mjesto i datum
Zagreb, Hrvatska, 13.10.2016. - 14.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Inflammatory bowel disease, gut microbiota

Sažetak
Inflammatory bowel disease (IBD) is a chronic gastrointestinal disorder with increased incidence (1). The onset of the disease is mainly associated with the immune status, gut microbiota and food intake. The current scientific knowledge of specific triggers and diagnostic markers is limited ; therefore, new data on gut microbiota, inflammatory, endocrine and nutritional status are required to better understand the IBD pathophysiology and ultimately support stratification of patients and initiation of appropriate therapy. In the translational project Assessment of Microbiota, Inflammatory Markers, Nutritional and Endocrinological Status in IBD Patients (Acronym: MINUTE for IBD) we are exploring host-gut microbiota interactions in order to define novel strategies for the management of IBD. Biological samples (stool, intestine biopsies, blood) as well as food and quality of life questionaires from 40 newly diagnosed IBD patients and 20 non-IBD control individuals will be collected and analysed in the period 2014-2018. One of the aims of MINUTE for IBD project is to optimize sensitive methodology of microbiota composition determination via next generation sequencing. Here we present evaluation of different DNA extraction kits and OMNIgene.GUT system as part of the protocol for collection, storage and analysis of microbial composition in human faecal samples.

Izvorni jezik
Engleski

POVEZANOST RADA