Naslov
Protein isolates from flaxseed cake as cell culture media supplement

Autori
Slivac, Igor ; Logarušić, Marijan ; Radošević, Kristina ; Gaurina Srček, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Esib/2017 / - Graz : Acib GmbH, 2017, 170-170

Skup
European summit of industrial biotechnology

Mjesto i datum
Graz, Austrija, 14.11.2017. - 16.11.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
animal cells, protein isolates, medium

Sažetak
Animal cell culture has a great importance in production of recombinant proteins that are used for diagnostic, therapeutic and preparative purposes. For successful maintenance of animal cell culture, it is essential to ensure growth supporting microenvironment. The composition of culture media has a key role in this process, where standardly added animal serum has many drawbacks. To overcome this problem, cell culture experts developed serum-free media where addition of protein hydrolysates, preferably of plant origin, replace serum proteins. Amongst the most promising substances of hydrolysates sources are protein rich by- products of edible oil industry called oil cakes or oil meals. Oil-cakes of flaxseeds contain 30-35% proteins, and as such can be used as a nutritious media supplement for animal cell cultivation. The main objective of the proposed study is to determine the effects of flaxseed oil cake isolates on growth and productivity of CHO-RP cell line that produces recombinant protein. Also, the effects of protein isolates on growth of HEK-293T cell line was studied. Obtained results showed that the addition of protein isolates at concentrations of 0.1 g/L and 0.2 g/L had positive effects on growth and productivity of CHO-RP cells, where concentrations above 1 g/L showed inhibitory effects on both cell lines growth. These preliminary results indicated possible application of protein isolates from flaxseed meals as a beneficial culture media component.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA