Naslov
Manure fertilizer increases antibiotic resistance

Autori
Udiković Kolić, Nikolina ; Wichmann, Fabienne ; Handelsman, Jo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of microbes in industry and environment : Programme and abstracts / Mrša, Vladimir ; Teparić, Renata ; Kifer, Domagoj - Zagreb, 2016, 28-28

ISBN
978-953-7778-14-9

Skup
Power of microbes in industry and environment

Mjesto i datum
Krk, Hrvatska, 28.09.2016. - 01.10.2016

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antibiotic resistance ; manure, soil

Sažetak
The increasing prevalence of antibiotic resistant bacteria is a serious public health threat. One route by which resistance genes enter the food system is through amendment of soils with manure from farm animals, which are considered a reservoir of such genes. The objective of this study was to assess the impact of cow manure on the ß-lactam resistance profile and composition of bacterial communities in soil. We treated soil with either inorganic fertilizer (NPK) or manure from dairy cows with no history of antibiotic treatment. Culturing showed that the soil treated with manure contained a higher abundance of β-lactam resistant bacteria than soil treated with NPK. Functional metagenomics indicated that the higher levels of resistant bacteria in manure-amended soil was attributable to an enrichment of resident soil bacteria that harbor β-lactamases. Quantitative PCR showed that manure treatment enriched the bla(CEP-04) gene, which is highly similar (96%) to a gene found previously in a Pseudomonas sp. Analysis of 16S rRNA genes by 454 sequencing indicated that the abundance of Pseudomonas spp. increased in manure-amended soil. Populations of other soil bacteria that commonly harbor β-lactamases, including Janthinobacterium sp. and Psychrobacter pulmonis, also increased in response to manure treatment. Our results indicate that manure fertilization may lead to blooms of certain antibiotic resistant subpopulation of the soil community, even when the manure comes from cows that have not been treated with antibiotics. These data demonstrate the importance of nonintuitive impacts of agricultural practices on the intrinsic resistance of soil microbial communities.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Biotehnologija

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