Naslov
Determination of the Solvent Blue 35 dye in diesel fuel by solid phase extraction and high-performance liquid chromatography with ultraviolet detection

Autori
Tomić, Tatjana ; Babić, Sandra ; Biošić, Martina ; Uzorinac Nasipak, Nada ; Čižmek, Ana-Marija

Izvornik
Dyes and pigments (0143-7208) 150 (2018); 216-222

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diesel fuel ; dye ; Solvent Blue 35 ; Euromarker ; solid phase extraction ; liquid chromatography

Sažetak
The method for the determination of blue dye Solvent Blue 35 (SB 35) in diesel fuel by high performance liquid chromatography (HPLC) coupled with UV-VIS detection was developed. The dye is separated from the hydrocarbon sample matrix and determined as a single component at 650 nm wavelength. Solid phase extraction (SPE) on the silica based solid phase sorbent was used as a pre-treatment method. Among investigated elution solvent mixtures, the mixture composed of chloroform and heptane in the volume ration of 1:0.75 (v/v) provided the best SB 35 recovery. During method validation two calibration models were tested: external calibration and matrix-matched calibration. For both models correlation coefficients were higher than 0.99 and slopes were compared using the Student's t-test. Precision was evaluated as repeatability and intermediate precision with relative standard deviations less than 7%. Low limit of quantification of 0.1 mg L−1 were obtained. Optimized procedure using 250 mg of sorbens provided recoveries ranged from 76.9% to 99.4% over a linear range (0.1–10 mg L−1). The developed method has been applied to the analysis of blue diesel samples from the market and suspicious samples. The content of SB 35 dye in a commercial blue diesel samples was found to be around 0.5 mg L−1 while presence of SB 35 dye in suspicious sample was confirmed.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne tehničke znanosti

POVEZANOST RADA