Naslov
Characterisation of antigen presenting cells in the human first trimester decidua

Autori
Štrbo, Nataša ; Laškarin, Gordana ; Sotošek Tokmadžić, Vlatka ; Bogović Crnčić, Tatjana ; Ćupurdija, Kristijan ; Juretić, Koraljka ; Dorčić, Dorotea ; Dupor, Jana ; Randić, Ljiljana ; Rukavina, Daniel ; Podack, Eckhard R.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th Congress of the Alps Adria Society for Immunology of Reproduction ; u: American Journal of Reproductive Immunology 48 (2002) (3) 153-160 ; P-19 / - , 2002, 160-160

Skup
Congress of the Alps Adria Society for Immunology of Reproduction (8 ; 2002)

Mjesto i datum
Weimar, Njemačka, 07.09.2002. - 10.09.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dendritic cell; decidua; pregnancy

Sažetak
Antigen presenting cells (APCs) are specifically equipped to initiate and maintain immune response. Among APCs, bone marrow-derived dendritic cells (DCs) are the most potent activators of naive T-lymphocytes responses. Decidual tissue is composed predominantly of typical stromal-type cells as well as glandular cells and leukocytes (mostly CD56+CD16- perforin bright + cells). The aim of this study was to determine phenotype of APCs present in the different culture conditions of human first trimester decidua as well as their interaction with decidual NK cells. Studies were performed on freshly isolated or cultured decidual cells (adherent and non adherent fraction). Phenotype of decidual adherent cells (DAC) was analysed by flow cytometry and immunocytochemistry. HLA-DR depleted and HLA-DR non depleted cells were used as possible modulators of perforin (P) expression and cytotoxicity of decidual lymphocytes (DL). We have found that DAC are heterogeneous cell population: 20% of DAC are CD45+ cells and 80% of DAC are CD45 negative and at the same time vimentin+, citokeratin-,  -actin+ and epidermal growth factor receptor (EGFR) +, indicating that they are decidual stromal cells. We have identified CD83+ DCs in adherent as well as nonadherent cell fraction of cultured decidual cells. It has been proven that CD83 is a suitable and selective cell-surface marker for mature DCs. Mature DC express HLA-DR as well as CD86 surface molecule. Contrary to DAC, the HLA-DR depleted DAC can not prevent the lost of perforin in DL. Decidual dendritic cells (CD83+) are suitable candidate that mediate the balance of maternal defensive immune responses to foreign antigens but also tolerance to the conceptus in the human decidua through interaction with decidual NK cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA