Naslov
Interaction of progesterone and IL-15 in the regulation of perforin expression and cytolytic activity of decidual lymphocytes at the materno-fetal interface

Autori
Laškarin, Gordana ; Štrbo, Nataša ; Sotošek-Tokmadžić, Vlatka ; Bogović-Crnčić, Tatjana ; Ćupurdija, Kristijan ; Juretić, Koraljka ; Dorčić, Dorotea ; Dupor, Jana ; Randić, Ljiljana ; Rukavina, Daniel ; Podack, Eckhard R.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th Congress of the Alps Adria Society for Immunology of Reproduction ; u: American Journal of Reproductive Immunology 48 (3) / - , 2002, 160-160

Skup
Congress of the Alps Adria Society for Immunology of Reproduction (8 ; 2002)

Mjesto i datum
Weimar, Njemačka, 07.09.2002. - 10.09.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
first trimester pregnancy ; decidua ; perforin ; IL-15

Sažetak
Human first trimester pregnancy decidua, characterised by high local concentration of progesterone, is infiltrated by decidual lymphocytes (DL) and HLA-DR+ cells, mostly macrophages. Predominant lymphocyte population are decidual non-classical NK cells. These cells are CD45+CD56bright+ and contain high level of perforin (P) in their granules. IL-15, as a main NK differentiation and proliferation factor is highly expressed in decidua and secreted by HLA-DR+ cells. Our previous results showed that progesterone in high concentration downregulates the number of P+ cells, as well as the content of P per cell, while IL-15 upregulates P expression in cultured DL. The aim of this study was to investigate possible role of progesterone in regulation of IL-15 induced cytotoxicity of DL. The suspension of freshly isolated decidual cells was incubated 18 or 72 hrs with progesterone, IL-15 or combination and cytolytic activity of DL was measured against NK sensitive K562 cells in 2 hrs cytotoxicity PKH26 (red) test. Concanymicin A pre-treatment was used to assess the contribution of perforin-mediated cytotoxcitiy. Intracellular content of perforin and IL-15 was measured by flow cytometry. We found that IL-15 on a dose dependent manner increase cytolytic activity of DL. Cytolytic activity of IL-15 stimulated DL for 72 hrs against K562 cell line was almost completely abrogated by Concanamycin A. Progesterone decreases cytolytic activity of DL and the percentage of IL-15+/HLA-DR+ cells in the culture. IL-15 reverted progesterone mediated suppression of cytolytic activity of DL against K562 cells. Conclusion: Our results clearly show that progesterone affects perforin mediated cytotoxcitiy of DL, as well as expression of IL-15 by decidual HLA-DR+ cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
Germany Conference venue : 'neue weimarhalle'.

POVEZANOST RADA