Naslov
The presence and regulation of IL-15 protein expression in human first trimester pregnancy decidua

Autori
Laškarin, Gordana ; Sotošek Tokmadžić, Vlatka ; Bogović, Tatjana ; Ćupurdija, Kristijan ; Vlastelic, Ivan ; Randić, Ljiljana ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Godišnji sastanak HID-a 2001 : Knjiga sažetaka / - Zagreb : Hrvatsko imunološko društvo, 2001, 8-8

Skup
Godišnji sastanak HID-a

Mjesto i datum
Zagreb, Hrvatska, 07.12.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
IL-15; Decidua; pregnancy; progesterone/PIBF; IL-15 expression; macrophages

Sažetak
During healthy pregnancy the dominance of Th2 cytokine production is found in peripheral blood and at maternal-fetal interface. Progesterone and soluble mediator of its function Progesterone Induced Blocking Factor (PIBF) induced Th2 cytokine production in already established Th1 decidual and peripheral blood clones of pregnant women. Th2 cytokine production is accompanied by attenuated NK cell cytotoxicity and are favourable for the gestation. Th1 cytokines support cell mediated immunity and are elevated during pregnancy loss. However, Th1 cytokine IL-15 mRNA was found in normal mouse and human decidua. Decidual NK cells proliferate, accumulate perforin and increase cytotoxicity under IL-15 stimulation in vitro. It is presumed that IL-15 induce proliferation of decidual CD3-CD16-CD56+ NK cells and accumulate cytolytic protein-perforin in their granules in vivo. The aim of this study was to analyse the presence and regulation of IL-15 protein expression in human first trimester pregnancy decidua. Immunohistology of frozen or paraffin embedded first trimester normal human pregnancy decidua was used for IL-15 protein detection. Decidual mononuclear cell, isolated by enzymatic digestion of decidual tissue and gradient density centrifugation were cultured 18 hours in the medium only or in the presence of progesterone (10  g/ml) or PIBF (5  g/ml). Nonadherent cells were removed. Decidual adherent cells (DAC) were collected from the bottom of Petri dishes, stimulated with PMA, Ionomycin and monenzin for further 4 hours, double labelled by anti-IL-15 and anti-HLA-DR antibody and analysed by flow cytometry. Smooth muscle cells of decidual vessels and subdecidual myometrium, glandular cells and cells distributed over the stroll were IL-15+ by immunohistology. Flow cytometry showed 35% of IL-15+ cells in the suspension of DAC, among which 15% were IL-15+/HLA-DR+ (macrophages) and 20% IL-15+/HLA-DR- (stromal) cells. Progesterone and PIBF significantly decreased percentage of IL-15+ cells in the suspension of DAC, due to decrease of IL-15 expression in decidual macrophages. Although progesterone/PIBF in vitro decrease IL-15 expression in decidual HLA-DR+ cells, probably macrophages, there are many IL-15+ cells in first trimester pregnancy decidua characterised by high concentration of progesterone. This results indicate the presence of potent IL-15 stimulator in decidual tissue in vivo.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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