Naslov
The presence and role of interleukin-18 at maternal-fetal interface

Autori
Sotošek Tokmadžić, Vlatka ; Tsuji, Y. ; Bogović, Tatjana ; Laškarin, Gordana ; Ćupurdija, Kristijan ; Štrbo, Nataša ; Randić, Ljiljana ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Godišnji sastanak HID-a 2001 : Knjiga sažetaka / - Zagreb : Hrvatsko imunološko društvo, 2001, 3-3

Skup
Godišnji sastanak HID-a

Mjesto i datum
Zagreb, Hrvatska, 07.12.2001

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytolytic activity; decidual lymphocytes; IL-18; perforin; IL-18R

Sažetak
First trimester human decidual is abundantly infiltrated with uterine NK cells (uNK) of unusual phenotype (CD3-CD16-CD56bright+) that contain high amount of cytolytic protein perforin in their granules. During early pregnancy uNK cells are exposed to many cytokines that influence their cytolytic activity. Recently discovered IL-18, as a multifunctional cytokine, participates in regulation of both Th1 and Th2 responses and augment both innate and acquired immunity. IL-18 is described as potent inducer of cytolytic activity of NK cells. Since there are very few data about IL-18 expression during pregnancy, the aim of this study was to analysed the presence of IL-18 and IL-18 receptor (IL-18R) at maternal-fetal interface and the possible role of IL-18 on perforin expression and NK cytotoxicity of decidual lymphocytes (DL). Decidual tissue of first trimester pregnancy decidua was enzyme digested and decidual cells from Lymphoprep interface was stimulated overnight with IL-18 (10 ng/ml) or cultured in the medium only. Cytolytic activity of non-adherent cells (DL) against K-562 targets was measured in 2 hr PKH-26 (red) cytotoxicity assay by flow cytometry. Decidual adherent cells (DAC) were used for intracellular staining of IL-18. The number of perforin positive DL, separated from DAC, were cultured for 18 hrs with IL-18 (5, 10, 15 or 20 ng/ml) or the medium only and analysed by flow cytometry. IL-18 and IL-18R expression on the trophoblast cells was detected by immunohistology. IL-18 added in a dose of 10 ng/ml upregulates perforin protein expression and cytolytic activity of DL. Approximately 20% of DAC are IL-18 positive and this percentage could be upregulated by PMA and Ionomycin stimulation. Double labelling of DAC with anti IL-18 and anti HLA-DR monoclonal antibody showed only about 3% of cells are at the same time double positive. By immunohistology IL-18R positive cells were found on syncytiotrophoblast cell layer, interstitial tissue cells of villi and fetal blood cells. There was no detectable IL-18 staining on trophoblast cell layer on villi, but strong staining of fetal blood cells in villous vessels. These are first results showing IL-18R expression, but not IL-18 expression on villous trophoblast cells, as well as enhancement of perforin expression and NK cytolytic potential of DL under the influence of IL-18. IL-18 in concert with other cytokines and hormones could play an important role in the regulation of cytolytic potential of first trimester pregnancy decidual NK cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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