Naslov
The role of perforin mediated cytolytic pathway in the pathophysiology of psoriasis

Autori
Prpić, Larisa ; Laškarin, Gordana ; Sotošek, Vlatka ; Gruber, Franjo ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 9th Congress European Academy of Dermatology & Venerology (EADV) ; u: JEADV 14 (2000) (S1) / - Ženeva, 2000, 253-253

Skup
Congress of the European Academy of Dermatology & Venerology (9 ; 2000)

Mjesto i datum
Ženeva, Švicarska, 11.10.2000. - 15.10.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
psoriasis; cell mediated cytotoxicity; NK mediated cytotoxicity; perforin; peripheral blood lymphocytes

Sažetak
Psoriasis represents an autoimmune inflammatory skin disease characterized by profound immunological changes, both at systemic and local level. It has been suggested that cellular interactions and fluctuations in the psoriatic skin of the different inflammatory cells emphasizes fluctuating nature of the disease i.e. exacerbation and remission. The role of cytolytic lymphocytes, particularly cytotoxic T lymphocytes (CTL) is recognized, but generally the role of cytolytic mechanisms at the molecular level is almost completely unknown. The aim of the investigation was to highlight the role of perforin (P) mediated cytotoxicity in the pathophysiology of the disease. Perforin is cytolytic molecule expressed in the granules of cytolytic cells both CTL and NK cells. Psoriatic patients in exacerbation and remission phase of chronic psoriasis and healthy persons, corresponding by age and sex, to psoriatic group, were investigated. Peripheral blood lymphocytes (PBL) were tested either by single or double staining, for simultaneous detection of P (intracellular antigen) and cell surface antigens by cell permeabilization method and flow cytometric analyses. NK cytotoxicity was tested against K-562 cell line by using 2 hours PKH-26 test. Perforin expression in the cells infiltrating skin caught by psoriatic process was detected by immunohistochemistry. Single staining analyses showed a marked increase of total P+ cells, CD3+ and CD8+ lymphocytes in the PBL in the phase of exacerbation of the disease. This was accompanied by significant increase of double positive cells (CD3+P+ and CD8+P+) and the frequency of P+ cells among the cells of CD16+ phenotype. Average fluorescence intensity (AFI) for P+ cells showed lower P values in exacerbation than in remission, but they both were regularly lower than in healthy control. NK cytotoxicity was suppressed and immunohistochemistry showed infiltrations of P+ cells in dermis in the phase of exacerbation. These results are confirmatory and clearly point to the potential participation of perforin mediated cytolytic pathway in the clinical course of disease.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija

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