Pregled bibliografske jedinice broj: 91945
Membrane phenotype and expression of perforin and serine esterases by CD3- peripheral blood and decidual granular lymphocyte-derived clones
Membrane phenotype and expression of perforin and serine esterases by CD3- peripheral blood and decidual granular lymphocyte-derived clones // Periodicum Biologorum / Vitale, Branko (ur.).
Zagreb: Hrvatsko prirodoslovno društvo, 1996. str. 62-62 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 91945 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Membrane phenotype and expression of perforin and serine esterases by CD3- peripheral blood and decidual granular lymphocyte-derived clones
Autori
Gudelj, Lea ; Christmas, Stephen E. ; Laškarin, Gordana ; Johnson, Peter M. ; Rukavina, Daniel ; Podack, Echkard R.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Periodicum Biologorum
/ Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1996, 62-62
Skup
Third international Meeting "Mechanisms in Local Immunity"
Mjesto i datum
Opatija, Hrvatska, 25.09.1996. - 28.09.1996
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
CD3- clones; decidual granular lymphocytes; perforin; peripheral blood lymphocytes; serine esterases
Sažetak
Problem: Human first-trimester pregnancy decidua was found to contain large numbers of perforin (P)-containing cells, which varied in their membrane antigen phenotype. Here we report results obtained by analyzing CD3  clones derived from human early pregnancy decidua and peripheral blood. Method: Decidual tissue was obtained from vaginal termination of first trimester normal human pregnancies. CD3- clones were generated by limiting dilution cloning, following depletion of CD3+ lymphocytes. Cell membrane phenotype was determined by flow-cytometry. Perforin was detected by FACS analysis of permeabilised cells. Serine esterases (SE) were identified by histochemical staining for BLT-esterase. Results: Cloned decidual cell populations retained the overall antigenic phenotype of freshly isolated decidual NK-like cells. All CD3  clones, either derived from decidua or from peripheral blood contained perforin. Serine esterases were present in every decidual clone analysed. Conclusions: Limiting dilution cloning allows the clear-cut analysis of homogenous subsets of decidua derived NK-like clones. The presence of large amounts of perforin in all of the CD3-clones underlines the extensive transcription of perforin gene by NK-like lymphocytes.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
