Pregled bibliografske jedinice broj: 91939
Phenotype and function of human decidual leukocytes
Phenotype and function of human decidual leukocytes // Periodicum Biologorum / Vitale, Branko (ur.).
Zagreb: Hrvatsko prirodoslovno društvo, 1996. str. 48-48 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 91939 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Phenotype and function of human decidual leukocytes
Autori
Chrismas, Stephen E. ; Gudelj, Lea ; Deniz, G. ; Brew, R. ; Rukavina, Daniel ; Johnson, Peter M
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Periodicum Biologorum
/ Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1996, 48-48
Skup
Third International Meeting "Mechanisms in Local Immunity"
Mjesto i datum
Opatija, Hrvatska, 25.09.1996. - 28.09.1996
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Human decidualized endometrium; DGL; PBNK cells; HLA-g; KIRs
Sažetak
Human decidualized endometrium contains a high proportion of granular leukocytes (DGL) of predominant phenotype CD2+CD3-CD4-CD7+CD8-CD16-CD56bright+ that are generally less cytotoxic against standard natural killer (NK) cell targets than are peripheral blood NK (PBNK) cells. The purpose of this study was to determine any phenotypic differences between DGL and PBNK cells which might explain their differing cytotoxic specificities. Fresh DGL were obtained by collagenase digestion of decidual tissue from elective first trimester terminations of pregnancy. DGL and PBNK clones were derived by limiting dilution following phytohemagglutinin stimulation and growth in interleukin-2 (IL-2). Both PBNK and dGL clones retained a similar phenotype to that of fresh cells, although most of the latter lost expression of CD7. DGL clones differed from PBL clones in being mostly CD16-CD103+. Cytotoxic function of both CD8-CD16- and CD8-CD16+ DGL clones but not CD8+ DGL clones was inhibited by target cell expression of HLA-g. DGL clones produced varying amounts of IFN-gamma, GM-CSF, IL-2, IL-6 and IL-10. Fewer DGL than PBNK clones expressed CD11b (CR3) and the killer inhibitory (KIR) molecules CD94 and the p58 molecule recognized by the HP3E4 mAb. Most fresh DGL expressed cytoplasmic CD3-zeta but not cytoplasmic CD3-epsilon, but the latter was induced in most DGL clones. Differences in patterns of cell surface expression of KIRs but not lack of the signal transducing molecule CD3-zeta may explain the differing cytotoxic specificities of DGL and PBNK cells.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
