Naslov
Complete mitogenome sequence of Lagomorphs from Upper Palaeolithic in the Balkan refuge

Autori
Brajkovic, Vladimir ; Radović, Siniša ; Brajković, Dejana ; Girardi, Matteo ; Krebs, Stefan ; Međugorac, Ivica ; Curik, Ino ; Miracle, T Preston ; Vernesi, Cristiano ; Cubric-Curik, Vlatka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 36th International Society for Animal Genetics Conference / - Dublin : International Society for Animal Genetics, 2017, 81-81

Skup
36th International Society for Animal Genetics Conference

Mjesto i datum
Dublin, Irska, 16.07.2017. - 21.07.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ancient DNA ; mtDNA ; haplotype ; genome sequencing ; diversity

Sažetak
Ancient DNA (aDNA) analysis is an important tool capable in resolving numerous questions related to the evolution and adaptation of animals and plants. Lagomorph phylogenetic has several open topics. Here, our interests referred to European rabbit (Oryctolagus cuniculus) where the Iberian Peninsula is considered as a main reservoir of genetic diversity as well as to migration dynamics and relation between brown hare (Lepus europaeus) and mountain hare (Lepus timidus) with respect to single (Balkans) versus multiple (Mediterranean Basin) refuges hypotheses. We have started our analysis with ancient bones of 54 lagomorphs, from deposits dated to different archaeological periods between the Upper Palaeolithic to Roman Period, that were taken from eight different archaeological sites in Croatia. After aDNA extraction, samples were prepared by multiplexed target capture enrichment method and genotyping by next-generation-sequencing (MiSeq Illumina Sequencing Platform). Complete mitogenome (around 16291 bp) was obtain for five lagomorphs that, according to taxonomic identification, exclusively based on morphometric and morphologic characteristics of bones, were previously classified as brown (2) and mountain (1) hares as well as rabbits (2). When phylogenetic analysis were performed with all complete mitogenome sequences available in the GenBank belonging to Lepus sp. and Oryctolagus cuniculus following results were obtained: (1) two different unique brown hare haplotypes were grouped with other brown hare haplotypes from Germany, Greece, Poland and Sweden ; (2) unique mountain hare haplotype was in branches with mountain hare haplotypes from Finland and as well as with Lepus granatensis from Spain and (3) two unique haplotypes were identified as typical Oryctolagus cuniculus haplotypes. Despite their chronostratigraphic correlation within archaeological layers, additional radiocarbon dating of successful samples is required, which is of particular interest for better understanding of rabbit origin.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Poljoprivreda (agronomija), Arheologija

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