Naslov
Effect of grape seed proanthocyanidins on retinoic acid-induced bone loss in rats

Autori
Oršolić, Nada ; Nemrava, Johann ; Kukolj, Marina ; Odeh, Dyana ; Lesar, Nikola ; Kunštić, Martina ; Goluža, Eleonora ; Jeleč, Željko ; Vihnanek Lazarus, Maja ; Orct, Tatjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2017 Annual Meeting of the Croatian Immunological Society with EFIS on Tour / - , 2017, 56-56

Skup
2017 Annual Meeting of the Croatian Immunological Society with EFIS on Tour

Mjesto i datum
Zagreb, Hrvatska, 20.10.2017. - 21.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Proanthocyanidins, osteoporosis, oxidative stress

Sažetak
Osteoporosis is a skeletal disease characterized by the significant decrease in bone mineral density (BMD) and bone microarchitecture disorder. The risk of osteoporosis can be genetic or acquired. Contributing to acquire risk are numerous medications, including 13-cis retinoic acid (13cRA). The main effects of 13cRA that contribute to bone loss are: a) increased oxidative stress (OS) and the formation of reactive radicals, decreased activity of osteoblasts and increased osteoclast activity ; b) osteoblasts apoptosis ; c) decreased levels of estrogen and appearance of inflammatory cytokines, d) reducing the activity of vitamin D receptor, decrease of the absorption of Ca2+ in the intestine, increasing excretion of calcium through the kidneys ; d) the effects on parathyroid hormone. Epidemiological evidence has indicated a link between dietary intake of antioxidants such as grape seed proanthocyanidins and bone health. The present study investigates the antiosteoporotic effects of grape seed proanthocyanidins (GSPs), natural antioxidants, in a 13cRA-induced osteoporosis model of rats and healthy rats. Three month-old female rats of the Y59 strain were given GSPs (100 mg kg-1) or alendronate (AL ; 40 mg kg-1, a positive control) concomitant with 13cRA (80 mg kg-1) once daily for 14 days by a single intragastric (i.g.) application. Twenty four hours after the treatment, we analysed bone turnover serum biochemical markers, such as: osteocalcin (OC), C- terminal fragment of type I collagen (CTX), bone mineral density (BMD), bone ash contents, calcium (Ca) and phosphorus (P) content, geometrical and physical parameters of bone as well as oxidative stress parameters (glutathione levels and lipid peroxidation). 13cRA successfully induced osteoporosis-like changes in rats. The treatment with GSPs increased BMD, OC level, femoral geometric characteristics, Ca and P content (P=0.025 ; P=0.025) in 13cRA-induced bone loss model. In addition, GSPs-treated rats had significantly lower serum alkaline phosphatase activity (P<0.001) and malondialdehyde (MDA) levels in both liver and kidney (P<0.05). Histological results showed its protective action as well, through promotion of bone formation. The beneficial role of GSPs against the 13cRA-induced bone loss in rats indicates it could have similar protective action on bone health in humans and be an effective replacement for AL.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA