Naslov
A Gel-Chromatographic and Light Scattering Study of the Salmonella typhi Endotoxin

Autori
Deželić, Gjuro ; Deželić, Nada ; Jušić, Dubravka ; Pende, Berislav ; Sinković, Dagmar ; Zebec, Mirna

Izvornik
Croatica chemica acta (0011-1643) 49 (1977), 1; 149-162

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Salmonella typhi endotoxin: Gel-cromatography ; Light scattering

Sažetak
The endotoxin of Salmonella typhi, strain 0-901, was isolated by extraction with hypertonic (1 M) sodium chloride solutions and studied by gel-chromatography and light scattering methods. The gel-chromatographic separation was performed on Sepharose 2B, Sepharose 4B and Sephadex G-200 gels, and the fractionated material was monitored by ultraviolet and phenol-sulfuric acid colorimetry as well as by a photometric latex agglutination test. The extracted material consisted of two components: one was the high molecular weight endotoxin and the other a protein-polysaccharide complex of a molecular weight lower than 66, 000 dalton. The light scattering experiments of endotoxin extracts showed the average molecular weights from 1.9 to 4.9 million dalton. The separation of the low molecular weight proteinic component was attempted by thermal denaturation, but this had to be abandoned owing to the denaturation and degradation of endotoxin. A high molecular weight endotoxin component was isolated by elution on a Sephadex G-200 column and had the molecular weight of 5.6 million dalton, which was in good accord with the value previously determined for a Boivin extraction sample. The high molecular weight endotoxin sample proved to be a highly polydispersed material. From the estimates of various averages of gyration radii it has been concluded that the particles of this sample have a more compact structure than those of the Boivin extraction sample, possibly due to the tertiary structuring of polypeptide chains in the proteinsaccharide complex of the endotoxin particle.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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