Naslov
Application of membrane filtration with the possibility of removing the LAB-AFM1 complex from milk

Autori
Jakopović, Željko ; Čanak, Iva ; Frece, Jadranka ; Bošnir, Jasna ; Kuharić, Željka ; Pavlek, Željka ; Ivešić, Martina ; Markov, Ksenija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Archives of industrial hygiene and toxicology 68: S57 / - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2017, 57-57

Skup
Food Safety and Quality Congress

Mjesto i datum
Opatija, Hrvatska, 21.11.2017. - 24.11.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aflatoxin elimination ; aflatoxin M1 ; food safety ; lactic acid bacteria

Sažetak
Previous scientific studies indicated a need for further development of applying biological methods in the removal / binding or biotransformation of mycotoxins in food and feed. Such methods should inactivate mycotoxins without affecting the nutritional value and organoleptic properties of the product being processed. In practice, synthetic and natural zeolites are most commonly used as fodder additives, which effectively eliminate aflatoxin through adsorption ; however, the drawback is that necessary nutrients are also being absorbed in the process. Therefore, the microbial population from fresh cow's milk and traditional dairy products such as cottage cheese and cream was isolated and identified with the aim of selecting bioprotective cultures as biofixator for the removal of AFM1 from milk. One hundred and fifty colonies of LAB were isolated and identified by API tests and ten strains were confirmed with molecular (PCR) methods. All of the confirmed strains (Lactobacillus plantarum SM1, Lactobacillus helveticus S9, Lactococcus lactis 5MS1, Lactobacillus plantarum SMB, Lactobacillus plantarum SMA, Lactobacillus plantarum SS1, Lactobacillus paracasei KM, Lactobacillus rhamnosus KM, Lactobacillus plantarum KM, Lactobacillus plantarum MM)) were tested for possible removal/binding of AFM1 from milk. Therefore, one of the goals of the study was to implement the practical application of membrane filtration, with the possibility of removing the complex biofixator-AFM1 from milk and preserving the nutritional value of milk. The amount of unbound AFM1 was determined by HPLC.

Izvorni jezik
Engleski

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