Pretraga

Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 910334

Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor

Mainali, Laxman; Sidabras, Jason W.; Camenisch, Theodore G.; Ratke, Joseph J.; Raguz, Marija; Hyde, James S.; Subczynski, Witold K.
Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor // Applied Magnetic Resonance, 45 (2014), 12; 1343-1358 doi:10.1007/s00723-014-0578-7 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 910334 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor

Autori
Mainali, Laxman ; Sidabras, Jason W. ; Camenisch, Theodore G. ; Ratke, Joseph J. ; Raguz, Marija ; Hyde, James S. ; Subczynski, Witold K.

Izvornik
Applied Magnetic Resonance (0937-9347) 45 (2014), 12; 1343-1358

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Eye lens ; Loop-gap resonator ; Membrane ; Saturation recovery ; Spin-label ; W-band

Sažetak
Spin-label W-band (94 GHz) EPR with a five-loop- four-gap resonator (LGR) was successfully applied to study membrane properties (L. Mainali, J.S. Hyde, W.K. Subczynski, Using spin-label W-band EPR to study membrane fluidity in samples of small volume, J. Magn. Reson. 226 (2013) 35-44). In that study, samples were equilibrated with the selected gas mixture outside the resonator in a sample volume ~100 times larger than the sensitive volume of the LGR and transferred to the resonator in a quartz capillary. A seven-loop-six-gap W-band resonator has been developed. This resonator permits measurements on aqueous samples of 150 nL volume positioned in a polytetrafluoroethylene (PTFE) gas permeable sample tube. Samples can be promptly deoxygenated or equilibrated with an air/nitrogen mixture inside the resonator, which is significant in saturation-recovery measurements and in spin-label oximetry. This approach was tested for lens lipid membranes derived from lipids extracted from two porcine lenses (single donor). Profiles of membrane fluidity and the oxygen transport parameter were obtained from saturation-recovery EPR using phospholipid analog spin-labels. Cholesterol analog spin-labels allowed discrimination of the cholesterol bilayer domain and acquisition of oxygen transport parameter profiles across this domain. Results were compared with those obtained previously for membranes derived from a pool of 100 lenses. Results demonstrate that EPR at W-band can be successfully used to study aqueous biological samples of small volume under controlled oxygen concentration.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Profili:

Avatar Url Marija Raguž (autor)

Poveznice na cjeloviti tekst rada:

doi pubmed.ncbi.nlm.nih.gov

Citiraj ovu publikaciju:

Mainali, Laxman; Sidabras, Jason W.; Camenisch, Theodore G.; Ratke, Joseph J.; Raguz, Marija; Hyde, James S.; Subczynski, Witold K.
Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor // Applied Magnetic Resonance, 45 (2014), 12; 1343-1358 doi:10.1007/s00723-014-0578-7 (međunarodna recenzija, članak, znanstveni)
Mainali, L., Sidabras, J., Camenisch, T., Ratke, J., Raguz, M., Hyde, J. & Subczynski, W. (2014) Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor. Applied Magnetic Resonance, 45 (12), 1343-1358 doi:10.1007/s00723-014-0578-7.
@article{article, author = {Mainali, Laxman and Sidabras, Jason W. and Camenisch, Theodore G. and Ratke, Joseph J. and Raguz, Marija and Hyde, James S. and Subczynski, Witold K.}, year = {2014}, pages = {1343-1358}, DOI = {10.1007/s00723-014-0578-7}, keywords = {Eye lens, Loop-gap resonator, Membrane, Saturation recovery, Spin-label, W-band}, journal = {Applied Magnetic Resonance}, doi = {10.1007/s00723-014-0578-7}, volume = {45}, number = {12}, issn = {0937-9347}, title = {Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor}, keyword = {Eye lens, Loop-gap resonator, Membrane, Saturation recovery, Spin-label, W-band} }
@article{article, author = {Mainali, Laxman and Sidabras, Jason W. and Camenisch, Theodore G. and Ratke, Joseph J. and Raguz, Marija and Hyde, James S. and Subczynski, Witold K.}, year = {2014}, pages = {1343-1358}, DOI = {10.1007/s00723-014-0578-7}, keywords = {Eye lens, Loop-gap resonator, Membrane, Saturation recovery, Spin-label, W-band}, journal = {Applied Magnetic Resonance}, doi = {10.1007/s00723-014-0578-7}, volume = {45}, number = {12}, issn = {0937-9347}, title = {Spin-label W-band EPR with Seven-Loop–Six-Gap Resonator: Application to Lens Membranes Derived from Eyes of a Single Donor}, keyword = {Eye lens, Loop-gap resonator, Membrane, Saturation recovery, Spin-label, W-band} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

Citati:

    Altmetrijski pokazatelji:


    Podijeli:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font
    CROSBI koristi kolačiće (cookies) kako bi poboljšao funkcionalnost stranice.