Naslov
Hemolysis of human erythrocytes provoked by bile acids

Autori
Čuka, Smiljana ; Mrakovčić-Šutić, Ines ; Dvornik, Štefica ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka / . - Zagreb, 2002

Skup
1st International Croatian Congress on Molecular Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
erythrocytes; bile acids

Sažetak
In the present pilot study we aimed to investigate their cytotoxicity to cellular membranes of red blood cells (RBC) monitoring the degree of hemolysis induced by different bile acids. The methodology of this study is based on spectrophotometric follow up of hemolysis after the addition of a small volume of RBC into a large excess of a defined isoosmotic solution (pH 7,5) containing bile acids. Sodium salts of cholic (CA), chenodeoxycholic (CDCA), deoxycholic (DCA) and lithocholic (LCA) acids were added in concentrations of 50, 250, 500 and 1000 mol/l to isoosmotic solution containing RBC. The results showed that LCA and CDCA were the most damaging to RBC provoking hemolysis in the range of 83,6-2781,6 mg/l and 133,8-1170,4 mg/l respectively. CA induced moderate hemolysis (41,8-585,7 mg/l), and DCA showed the lowest degree of hemolysis (50,2-334,4 mg/l). The hemolytic potency of bile acid proved to be dose-related. The correlation between the hemolysis and individual bile acid concentration was high (CA 0,975, CDCA 0,988, DCA 0,970, LCA 0,984), and statistically significant (P<0,05). These results imply that the presence of bile acids can cause damage of cell membrane that might explain complex pathogenesis of several clinical disorders that appear during cholestasis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

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