Naslov
Preanalytical aspects influences immunocytochemistry result in pulmonary samples

Autori
Harabajsa, Suzana ; Jonjić, Nives ; Juroš, Zrinka ; Vrabec Branica, Božica ; Smojver-Ježek, Silvana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Skup
40th European congress of cytology

Mjesto i datum
Liverpool, Ujedinjeno Kraljevstvo, 02.10.2016. - 05.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Preanalytical aspects ; immunocytochemistry ; pulmonary cytology
(40th European congress of cytolog)

Sažetak
Objective: To present the preanalytical aspects of immunocytochemistry (ICC) procedure in pulmonary cytology samples in our institution. Materials and methods: Retrospective analysis included 428 ICC samples processed in one year in Division of Pulmonary Cytology. Among 428 samples were 100 (23, 36%) bronchial washings (BW) ; 118 (27, 57%) bronchial brushings (BB) ; 18 (4, 21%) bronchoalveolar lavage fluids (BAL) and 41 (9, 58%) transbronchial fine needle aspirations (TBFNA) obtained during bronchoscopies. Among others, were 62 (14, 49%) lymph node FNAs (LN) ; 31 (7, 24%) transthoracic fine needle aspirations (TTFNA) and 58 (13, 55%) pleural effusions (PE). Pulmonologists and radiologists obtained all samples except lymph nodes FNAs. Before ICC, unstained slides were microscopically evaluated for optimal number of malignant cells. Influence of the sample type, number of malignant cells, absence of staining and the sample desquamation during ICC process on the final ICC report were analysed. Results: Among 428 ICC samples, 39 (9, 11%) were inadequate for interpretation. Inadequate samples included 5/39 (12, 82%) BW ; 7/39 (17, 95%) BB ; 1/39 (2, 56%) BAL ; 5/39 (12, 82%) TBFNA ; 12/39 (30, 77%) PE ; 5/39 (12, 82%) LN and 4/39 (10, 26%) TTFNA. Among 31/39 (79, 49%) samples with insufficient number of malignant cells were 4/31 (12, 90%) BW ; 5/31 (16, 13%) BB ; 1/31 (3, 23%) BAL ; 4/31 (12, 90%) TBFNA ; 11/31 (35, 48%) PE ; 3/31 (9, 68%) LN and 3/31 (9, 68%) TTFNA. Staining was absent in 5/39 (12, 82%) samples including 1/5 BW ; 2/5 BB ; 1/5 PE and 1/5 LN. Among 3/39 (7, 69%) desquamated samples were one TBFNA, LN and TTFNA. Conclusion: Pleural effusion was a sample with the highest percentage of unsuccessful ICC due to suboptimal preanalytical procedure. Optimal dilution of antibodies prevents the absence of staining. Routine usage of adequate cytological slides may prevent sample desquamation. Additional microscopic examination of unstained slides is helpful in minimising number of inadequate samples for ICC.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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