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Pregled bibliografske jedinice broj: 909494

Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB

Weibezahn, Jimena; Tessarz, Peter; Schlieker, Christian; Zahn, Regina; Maglica, Zeljka; Lee, Sukyeong; Zentgraf, Hanswalter; Weber-Ban, Eilika U.; Dougan, David A.; Tsai, Francis T.F. et al.
Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB // Cell, 119 (2004), 5; 653-665 doi:10.1016/j.cell.2004.11.027 (podatak o recenziji nije dostupan, članak, znanstveni)

CROSBI ID: 909494 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB

Autori
Weibezahn, Jimena ; Tessarz, Peter ; Schlieker, Christian ; Zahn, Regina ; Maglica, Zeljka ; Lee, Sukyeong ; Zentgraf, Hanswalter ; Weber-Ban, Eilika U. ; Dougan, David A. ; Tsai, Francis T.F. ; Mogk, Axel ; Bukau, Bernd

Izvornik
Cell (0092-8674) 119 (2004), 5; 653-665

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ClpB, AAA+ chaperone, protein disaggregation, thermotolerance

Sažetak
Cell survival under severe thermal stress requires the activity of the ClpB (Hsp104) AAA+ chaperone that solubilizes and reactivates aggregated proteins in concert with the DnaK (Hsp70) chaperone system. How protein disaggregation is achieved and whether survival is solely dependent on ClpB-mediated elimination of aggregates or also on reactivation of aggregated proteins has been unclear. We engineered a ClpB variant, BAP, which associates with the ClpP peptidase and thereby is converted into a degrading disaggregase. BAP translocates substrates through its central pore directly into ClpP for degradation. ClpB-dependent translocation is demonstrated to be an integral part of the disaggregation mechanism. Protein disaggregation by the BAP/ClpP complex remains dependent on DnaK, defining a role for DnaK at early stages of the disaggregation reaction. The activity switch of BAP to a degrading disaggregase does not support thermotolerance development, demonstrating that cell survival during severe thermal stress requires reactivation of aggregated proteins.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA

Ustanove:
Sveučilište u Rijeci - Odjel za biotehnologiju

Profili:

Avatar Url Željka Maglica (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Weibezahn, Jimena; Tessarz, Peter; Schlieker, Christian; Zahn, Regina; Maglica, Zeljka; Lee, Sukyeong; Zentgraf, Hanswalter; Weber-Ban, Eilika U.; Dougan, David A.; Tsai, Francis T.F. et al.
Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB // Cell, 119 (2004), 5; 653-665 doi:10.1016/j.cell.2004.11.027 (podatak o recenziji nije dostupan, članak, znanstveni)
Weibezahn, J., Tessarz, P., Schlieker, C., Zahn, R., Maglica, Z., Lee, S., Zentgraf, H., Weber-Ban, E., Dougan, D. & Tsai, F. (2004) Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB. Cell, 119 (5), 653-665 doi:10.1016/j.cell.2004.11.027.
@article{article, author = {Weibezahn, Jimena and Tessarz, Peter and Schlieker, Christian and Zahn, Regina and Maglica, Zeljka and Lee, Sukyeong and Zentgraf, Hanswalter and Weber-Ban, Eilika U. and Dougan, David A. and Tsai, Francis T.F. and Mogk, Axel and Bukau, Bernd}, year = {2004}, pages = {653-665}, DOI = {10.1016/j.cell.2004.11.027}, keywords = {ClpB, AAA+ chaperone, protein disaggregation, thermotolerance}, journal = {Cell}, doi = {10.1016/j.cell.2004.11.027}, volume = {119}, number = {5}, issn = {0092-8674}, title = {Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB}, keyword = {ClpB, AAA+ chaperone, protein disaggregation, thermotolerance} }
@article{article, author = {Weibezahn, Jimena and Tessarz, Peter and Schlieker, Christian and Zahn, Regina and Maglica, Zeljka and Lee, Sukyeong and Zentgraf, Hanswalter and Weber-Ban, Eilika U. and Dougan, David A. and Tsai, Francis T.F. and Mogk, Axel and Bukau, Bernd}, year = {2004}, pages = {653-665}, DOI = {10.1016/j.cell.2004.11.027}, keywords = {ClpB, AAA+ chaperone, protein disaggregation, thermotolerance}, journal = {Cell}, doi = {10.1016/j.cell.2004.11.027}, volume = {119}, number = {5}, issn = {0092-8674}, title = {Thermotolerance Requires Refolding of Aggregated Proteins by Substrate Translocation through the Central Pore of ClpB}, keyword = {ClpB, AAA+ chaperone, protein disaggregation, thermotolerance} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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