Naslov
Cyclodextrin encapsulation of daidzein and genistein by grinding: Implication on the glycosaminoglycan accumulation in mucopolysaccharidosys type II and III fibroblasts

Autori
Fumić, Barbara ; Jablan, Jasna ; Cinčić, Dominik ; Zovko Končić, Marijana ; Jug, Mario

Izvornik
Journal of microencapsulation (0265-2048) 35 (2018), 1; 1-12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
daidzein ; genistein ; cyclodextrin ; neat grinding ; glycosaminoglycans

Sažetak
This work was aimed to investigate the potential effect of cyclodextrin encapsulation on intrinsic ability of daidzein (DAD) and genistein (GEN) to inhibit the glycosaminoglycan (GAG) synthesis in fibroblasts originating from patients with mucopolysaccharidoes (MPS), type II and III. DAD or GEN encapsulation with either 2-hydroxypropyl-β-cyclodextrin or sulphobuthylether-β-cyclodextrin were achieved by neat grinding and characterised by thermal analysis, X-ray powder diffraction, scanning electron microscopy and solubility testing, confirming the complexes formation with increased solubility with respect to starting compounds. Both isoflavones, as well as their co-ground cyclodextrin complexes reduced GAG levels in the fibroblasts of MPS II and MPS III patient for 54.8-77.5 %, in a dose dependant manner, without any significant cytotoxic effect. Cyclodextrin encapsulation did not change the intrinsically high effect of both DAD and GEN on the GAG level reduction in the treated cells, thus could be considered as a part of combination therapies of MPS.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA