Naslov
Morfološka i proteomska analiza bičaša roda Trypanosoma na području Republike Hrvatske
(Morphological and proteomic analysis of flagellates from Trypanosoma genus in Croatia.)

Autori
Martinković, Franjo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 7th International Congress "Veterinary Science and Profession". / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb, 2017, 162-162

Skup
7th International Congress "Veterinary Science and Profession"

Mjesto i datum
Zagreb, Hrvatska, 05.10.2017. - 07.10.2017

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
morfologija, Trypanosoma, in vitro uzgoj, proteomska analiza
(morphology, Trypanosoma, in vitro cultivation, proteomic analysis)

Sažetak
The species of Trypanosoma genus are spread worldwide. Some species can cause serious health problems in humans and animals, although some species are apathogenic for their host. In Croatia apathogenic species were isolated: T. melophagium from sheep ked, T. theileri from cattle, Trypanosoma sp. from cervids and T. rotatorium from frogs. For seroepizootiological studies as antigen source, in vitro cultured trypanosomes are used. Except by in vitro cultivation, antigen research can be done with proteomic analysis. The aims of this study were to describe T. rotatorium bloodstream forms based on morphometric parameters, in vitro cultivation of isolated tripanosomes in new in vitro culture system medium as antigen source for further seroepizootiological research and their proteomic profiling. Also, by in vitro cultivation, goal is to describe the vitality and morphology of all the trypanosome species present in Croatia and culture the bloodstream forms for further seroepizootiological investigations. Proteomic analysis of trypanosome isolates will elucidate their differences and similarities. T. rotatorium morphology was described using standard morphometric parameters. A new in vitro culture, Biphasic Chocolate Agar for Trypanosomatids was compared with Liver Tryptose Medium by in vitro cultivation of isolated trypanosomes. Proteomic profile of trypanosomes was analyzed by two dimensional electrophoresis and mass spectrometry. Morphometric analysis of T. rotatorium bloodstream forms showed high polymorphism. By culturing the trypanosomes in new culture system, antigen for further seroepidemiological research was produced. Proteomic analysis showed marked difference between analyzed trypanosome flagellates. This is for the first time that T. rotatorium bloodstream forms were analyzed in Croatia. We showed here a new in vitro culture system media. For the first time proteome profile of T. rotatorium, T. theileri, T. melophagium and cervid trypanosome were presented.

Izvorni jezik
Hrvatski

POVEZANOST RADA