Naslov
UPORABA MOLEKULSKIH IN BIOKEMIJSKIH OZNAČEVALCEV ZA OPIS AVTOHTONIH PASEM DOMAČIH ŽIVALI
(THE USE OF MOLECULAR AND BIOCHEMICAL MARKERS IN THE DESCRIPTION OF AUTOCHTHONOUS BREEDS OF DOMESTIC ANIMALS)

Autori
Ivanković, Ante

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
BIOTEHNIŠKA FAKULTETA

Mjesto
Ljubljana, Slovenija

Datum
16.03

Godina
2001

Stranica
131

Mentor
Dovč, Peter

Neposredni voditelj
Dovč, Peter

Ključne riječi
molekularna genetika/osli/ovce/avtohtone pasme/proteinski polimorfizem/ mikrosatelitni lokusi/D-loop regija/PCR-SSCP/DNK sekvenčni polimorfizem
(molecular genetics/donkeys/sheep/autochthonous breeds/protein polymorphism/ microsatellite loci/D-loop region/PCR-SSCP/DNA sequence polymorphisms)

Sažetak
The genomes of Pag sheep and donkey subpopulations in Croatia. Were typed using several methodological approaches. In donkeys we estimated frequencies of transferrins and albumins in addition to typing of eight microsatellite loci. Genetic distances of donkey subpopulations based an to frequencies of polymorphic proteins match were the distances based on microsatellite typing. We sequenced 417 bp long fragment of the proksimal part of the D-loop region of donkey mtDNA. We found two basic sequence types within which several subtypes are present. The phylogenetic separation of two main sequences (X and Y) happened in a period 1, 2 to 3, 3 million years ago. We analysed the same segment of the D-loop region by PCR-SSCP and detected in addition to two main variants also an additonal subtype X'. In the Pag sheep breed we determined frequencies of hemoglobine, transferrin and albumin. We typed seven microsatellite loci and determined a high degree of variability. The average number of allele variants per locus was 8, 71. We sequenced 400 bp long fragment of the D-loop region. The existence of two basic types of mtDNA sequences was confirmed. The rarer sequence type (M) is genetically close to the sequence of Merino breed deposited in GenBank (AF010406) which draws to the conclusion that it might he a trace of the Merinisation of the Pag sheep. The other sequence type (N) belongs to the autochthonous Pag sheep. Frequencies of A and B variants of β -lactoglobulin (0, 5375 ; 0, 4625) and A and D variants of α s1 casein (0, 0875 ; 0, 1250) showed that by selection pressure on certain genotypes the efficiency of cheese production could be improved. These results are the first indicators for genetic profile of mentioned breeds and will stimulate further research and application of molecular data for selection.

Izvorni jezik
Slv

Znanstvena područja
Šumarstvo

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