Naslov
The effect of H2S-derived radical species in model lipid system

Autori
Tartaro Bujak, Ivana ; Mihaljević, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
30th Miller conference on radiation chemistry : book of abstracts / - , 2017, 55-55

Skup
Miller Conference 2017

Mjesto i datum
Castellammare del Golfo, Italija, 07.10.2017. - 11.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
H2S derived radicals, lipid peroxidation, geometrical isomerization

Sažetak
Hydrogen sulfide is endogenous gasotransmitter that is known to exert effects in cardiovascular and central nervous systems. Different H2S-derived radicals originating either naturally within the body or coming from the environment can be involved in inflammation during radical stress and in cell signaling. Generally, the double-sward character of thiol species towards biomolecules has been increasingly addressed in the last decade. There are two important characteristics of thiyl radicals: their ability to abstract hydrogen atoms from the molecules such as lipids, initiating lipid damage due to lipid peroxidation and to catalyse the double bond isomerization in polyunsaturated fatty acid. The reactions of thiyl radicals as isomerizing agents are not well known especially with the interplay of lipid peroxidation process. The aim of this study is to investigate the role of H2S as a precursor of diffusible simple S-centered radicals on lipid peroxidation and geometrical isomerization processes in biomimetic lipid model system. In this study lipid peroxidation via the formation of lipid hydroperoxides, and geometrical trans-isomerization were determined under biomimetic conditions. Thiyl radicals were generated by γ-radiation of simple model micellar system containing linoleic acid and H2S. Model lipid systems were irradiated up to the dose of 400 Gy, in equilibrium with air or after saturation with N2O at room temperature using panoramic 60Co source at the Ruđer Bošković Institute. Accurate dose rates in the irradiating positions were established with the ethanol-chlorobenzene dosimetry system (ISO/ASTM 51538:2009). The concentrations of hydroperoxides of linoleic acid were determined by the spectrophotometric ferric thiocyanate method and the geometrical isomerization of LH methyl ester was studied by capillary gas chromatography. In addition, we investigated the concentration effect of thiols on the outcome of the two lipid modification processes. We believe that this study could contribute to the understanding of H2S reactions which take place in biomembranes that could be essential to rationalize the biological properties of this newly recognized signaling molecule in biological systems.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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