Naslov
Praćenje i istraživanje proizvodnje biomase i sekundarnih metabolita entomopatogene gljive Beauveria brongniartii pomoću SPE-LC-PDA-ESI-Q- TOF-MS/MS i MALDI TOF/MS
(Monitoring and investigation of biomass and secondary metabolite production by entomophatogenic fungus Beaveria brongniartii by means SPE-LC-PDA-ESI-Q-TOF-MS/MS AND MALDI TOF-MS)

Autori
Kasal, Tina

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad

Fakultet
Poljoprivredni fakultet

Mjesto
Osijek

Datum
28.01

Godina
2009

Stranica
150

Mentor
Bešlo, Drago

Neposredni voditelj
Bešlo, Drago

Ključne riječi
secundary metabolites ; soil fungi ; extraction ; mass spectrometer

Sažetak
The monitoring of biomass production showed positive reaction on amount of proteins in media. The best media for biomass production were mSeed and Seed at temperatures of 20 and 25⁰C. Slow growth of strain shows very high amount of secondary metabolites. During investigation of secondary metabolites almost all detected compounds were completely new. From known compounds, Bassianolide, a compound known to be produced by Beauveria family, first time was detected to be produced by Beauveria brongniartii. Also for two other completely new compounds, bb_1 and bb_2, structural proposal was made. All compounds that have been produced in higher amount are non-polar. For future research, to identify other compounds it is necessary to perform a MS3 experiment for determination of fragments that it is impossible to identify only by MS/MS experiment. Farther more, for detection of exact position of all atoms in compound, NMR technology is needed. After isolation of certain compounds, biological tests are needed to prove entomolophatogenic abilities. Except testing it’s affiances on Melolontha melolontha, it is necessary to test Beauveriana brongniartii's effects on other insects and its effect on balance in nature.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti

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