Naslov
Functional ES-derived neuronal networks: a comparison with dissociated hippocampal neuronal networks

Autori
Ban, Jelena ; Bonifazi, Paolo ; Pinato, Giulietta ; Broccard, Frederic D ; Studer, Lorenz ; Torre, Vincent ; Ruaro, Maria Elisabetta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
EuroStemCell International Conference Advances in Stem Cell Research

Mjesto i datum
Lausanne, Švicarska, 08.09.2006. - 10.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ES cells ; In vitro differentiation ; Neuronal network ; Hippocampus

Sažetak
Embryonic stem (ES) cells provide a flexible and unlimited source for the derivation of various neuron types. One of the key attributes of neurons is the ability to establish neuronal networks. We tested whether ES derived neurons are capable of generating functional networks and whether such networks generated in vitro are capable of processing information. Intracellular recordings confirmed that ES- derived neurons spontaneously fire action potentials (APs) triggered by synaptic potentials. Extracellular recording with planar multielectrode arrays (MEAs) demonstrated bursts of spontaneous APs propagating throughout the entire network. The statistical properties of APs within such networks were remarkably similar to those of adult neurons. ES cell derived neuronal networks could discriminate between stimuli of different intensity at a single trial level, a key feature required for efficient information processing.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA