Pregled bibliografske jedinice broj: 904157
Hippocampal Filopodia but not Growth Cones can Climb Over 600 nm Steps
Hippocampal Filopodia but not Growth Cones can Climb Over 600 nm Steps // Biophysical Journal
Philadelphia (PA), Sjedinjene Američke Države, 2013. str. 162a-162a doi:10.1016/j.bpj.2012.11.914 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 904157 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Hippocampal Filopodia but not Growth Cones can Climb
Over 600 nm Steps
Autori
Lien, Linh Thuy ; Ban, Jelena ; Tormen, Massimo ; Migliorini, Elisa ; Grenci, Gianluca ; Pozzato, Alessandro ; Torre, Vincent
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Biophysical Journal
/ - , 2013, 162a-162a
Skup
Biophysical Society, 57th Annual Meeting
Mjesto i datum
Philadelphia (PA), Sjedinjene Američke Države, 02.02.2013. - 06.02.2013
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
guidance molecules ; growth cone
Sažetak
Guidance molecules, such as Sema3A or Netrin-1, can induce growth cone repulsion or attraction in the presence of a flat surface, but very little is known of the action of guidance molecules in the presence of obstacles. In order to address this issue, we analysed the action of Netrin-1 molecules in the presence of patterned polydimethylsiloxane (PDMS) surfaces with lines with a height varying from 100 to 600 nm. Filopodia can crawl over these lines easily, but not neurites. Indeed axons and thick dendrites are able to cross lines with a height of 100 nm, but progressively less when the line height is increased. When neurons are grown for 3 days over patterned surfaces it was possible to label selectively axons with axonal neurofilament marker SMI 312 and dendrites with microtubule associated protein 2 (MAP2): axons and dendrites do not differ in their ability to cross lines with a height varying from 100 to 600 nm. When axons and dendrites grow along lines, a clear staining for the adhesion marker paxillin was observed, but not when neurites cross the lines.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
