Naslov
Comparison of the Force Exerted by Hippocampal and Drg Growth Cones

Autori
Amin, Ladan ; Ercolini, Erika ; Ban, Jelena ; Torre, Vincent

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biophysical Journal / - , 2013, 477a-477a

Skup
Biophysical Society, 57th Annual Meeting

Mjesto i datum
Philadelphia (PA), Sjedinjene Američke Države, 02.02.2013. - 06.02.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
growth cones ; optical tweezers

Sažetak
Using optical tweezers, we have compared the force exerted by lamellipodia and filopodia from developing growth cones of isolated hippocampal neurons and Dorsal Root Ganglia (DRG) neurons obtained from P1-P2 and P10-P12 rats. Lamellipodia of DRG neurons were usually larger than those from hippocampal neurons and the proportion of filopodia with microtubules in their interior was higher in hippocampal than in DRG neurons. Filopodia from hippocampal growth cones exerted a large force often in the order of 5 pN, while the force exerted by filopodia of DRG growth cones was in the order of 1-2 pN and never exceeded 5 pN. Lamellipodia of both hippocampal and DRG growth cones exerted lateral forces up to 20 pN, but lamellipodia from DRG neurons could exert a vertical force larger than lamellipodia from hippocampal neurons. Therefore, the lateral force exerted by lamellipodia from peripheral nervous system (PNS) and central nervous system (CNS) neurons at different developmental stages is broadly similar but hippocampal filopodia could exerted a force larger than that of DRG filopodia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA