Naslov
Identifying Pex21p as a protein that specifically interacts with yeast seryl-tRNA synthetase

Autori
Ročak, Sanda ; Landeka, Irena ; Weygand-Đurašević, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Croatian Congress om Molecular Life Sciences with international participation / Dumić, Jerka - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2002

Skup
1st Croatian Congress om Molecular Life Sciences with international participation

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
seryl-tRNA synthetases; protein-protein interactions; Pex21p

Sažetak
Saccharomyces cerevisiae Pex21p is a peroxin involved in the peroxisome assembly (Purdue, P.E., Yang, X. and Lazarow, P.B. (1998) J. Cell Biol. 143, 1859-1869.) This protein also specifically interacts with yeast seryl-tRNA synthetase (SerRS), an essential enzyme required for serylation of tRNASer. SerRS/Pex21p protein interaction was identified by screening yeast cDNA library with SerRS as a bait in the two-hybrid system. The complex formation between these two proteins was also confirmed in vitro. Since eukaryotic aminoacyl-tRNA synthetases (aaRSs) are known to participate in assembles with other synthetases and non-synthetase proteins, we propose that the interaction with Pex21p may reflect another function of the peroxine, potentially related to the specificity of serylation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA