Naslov
Molecular imaging of cannabis leaf tissue with MeV-SIMS method

Autori
Jenčič, Boštjan ; Jeromel, Luka ; Ogrinc Potočnik, Nina ; Vogel-Mikuš, Katarina ; Kovačec, Eva ; Regvar, Marjana ; Siketić, Zdravko ; Vavpetič, Primož ; Rupnik, Zdravko ; Bučar, Klemen ; Kelemen, Mitja ; Kovač, Janez ; Pelicon, Primož

Izvornik
Nuclear instruments & methods in physics research. Section B : Beam interactions with materials and atoms (0168-583X) 371 (2016); 205-210

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Imaging mass spectrometry ; MeV-SIMS ; Molecular imaging ; THC ; THCA

Sažetak
To broaden our analytical capabilities with molecular imaging in addition to the existing elemental imaging with micro-PIXE, a linear Time-Of-Flight mass spectrometer for MeV Secondary Ion Mass Spectrometry (MeV-SIMS) was constructed and added to the existing nuclear microprobe at the Jožef Stefan Institute. We measured absolute molecular yields and damage cross-section of reference materials, without significant alteration of the fragile biological samples during the duration of measurements in the mapping mode. We explored the analytical capability of the MeV-SIMS technique for chemical mapping of the plant tissue of medicinal cannabis leaves. A series of hand-cut plant tissue slices were prepared by standard shock-freezing and freeze-drying protocol and deposited on the Si wafer. We show the measured MeV-SIMS spectra showing a series of peaks in the mass area of cannabinoids, as well as their corresponding maps. The indicated molecular distributions at masses of 345.5 u and 359.4 u may be attributed to the protonated THCA and THCA-C4 acids, and show enhancement in the areas with opened trichome morphology.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

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