Naslov
Fiziološke osobine i virulencija sojeva bakterije Staphylococcus hyicus
(Physiological properties and virulence of Staphylococcus hyicus strains)

Autori
Šeol, Branka

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Veterinarski fakultet

Mjesto
Zagreb

Datum
31.01

Godina
1997

Stranica
122

Mentor
Hajsig, Danko ; Župančić, Željko

Neposredni voditelj
Župančić, Željko

Ključne riječi
Staphylococcus hyicus; virulencija
(Staphylococcus hyicus; virulence)

Sažetak
Da bi se ustanovila učestalost nalaza bakterije S. hyicus pretraženi su materijali s kože i različitih sluznica zdravih krmača te kože zdravih krava, konja i pilića. S. hyicus izdvojen je samo s kože i različitih slunica krmača. Izdvojeni sojevi bakterije S. hycus su serotipizirani. Određene su biokemijske osobine sojeva bakterije S. hyicus a elektroforezom u poliakrilamidnom gelu određeni su proteinski profili cijelih stanica i supernatanata. In order to establish the frequency of the presence of Staphylococcus hyicus (S. hyicus) in several animal species, 165 materials originated from skin or different mucosa of 33 healthy sows were examined bacteriologicaly. In addition, 20 swabs sampled from skin of body and udder of healthy cows, 30 swabs from different parts of horse skin and 25 swabs taken from skin of healthy chickens were also examined. From 23 (69.7%) sows fifty two S. hyicus strains were isolated. The isolates were serotyped by slide agglutination test. The most frequent serovar was ATCC 19226 represented with 35 (67.3%) of isolated strains. Thirteen strains (25.0%) belonged to serovar ZH 1037 and four strains (7.7%) to serovar ZH 1029. Causative agent of exudative epidermitis in pigs (EEP) was the mostly represented on udders (13 sows), skin of snout and ears and nasal mucosa (11 sows), vaginal mucosa (nine sows) and conjunctivas (eight sows). From the skin swabs sampled from healthy cows, horses and chickens S. hyicus was not isolated. For all the S. hyicus strains investigated, including the control and the referent strains, biochemical and physiological profiles were determined. The referent strains consisted of all serovars of S. hyicus species, a strain isolated from horse and chicken, four strains isolated from cattle, strains marked as NCTC 10350 and 1403B-88 (originated from swine) were used as the control strains and obtained from different bacteriological strains collection in Western Europe. Biochemical profiles of all the strains analyzed were consistent except in urease activity and nitrate reduction. One strain originated from chicken did not fermented lactose and one control strain from cattle trehalose. Sodium dodecyl sulfate gel electrophoresis (SDS-PAGE) profile patterns of whole cells samples 29 epizooticaly unrelated strain showed great homology among strains of the same serovar. SDS-PAGE profile of sterile concentrated supernatant showed that five strains all ATCC 19926 serotype (strains marked as 26 A, 27 C, 27 E, 28 D and 29 D) isolated from healthy sows belong to separate group. Those strains contained a band corresponding to a protein with molecular weight of approximately 50 kDa and those of 45 kDa was not observed. Protein profile of sterile concentrated supernatant of control strain NCTC 10350 (control strain of high virulence) was different from the profile of the most swine strains and control strain 1402B-88 (control avirulent strain) and was very alike to profiles of five strains mentioned. A protein profile of the control strain marked as NCTC 10350 also differed from the most strains and was very similar to the profile of the five strains mentioned. It lacked the protein with molecular weight of 45 kDa and had the protein molecular weight of approximately 50 kDa. According to the presence of a protein with a molecular weight 50 kDa these five strains were considered as exfoliative toxin producing strains although the quantity of this bacterial toxin was not determined. However, in experimentally infected piglets inoculated with broth cultures of S. hyicus strains, till the very end of the experiment two of five strains mentioned did not cause visible changes on the skin of experimental piglet (strains marked as 27 C and 29 D) and three strains caused the occurrence of an abscess (strain marked as 28 D), an abscess with mild exfoliation (strain marked as 27 E) and localized exfoliation and exudation, respectively (strain marked as 26 A). The control virulent strain marked as NCTC 10350 caused the change typical for generalized kind of EEP on the skin of an experimental piglet. The strains originated from horse and chicken, respectively, did not cause visible changes on skin. Only one strain, out of four strains originated from cattle caused abscess and exfoliation in an experimental piglet. By the means of biological experiment carried out on chickens in 16 investigated S. hyicus strains the ability of producing the exfoliative toxin was investigated. Seven strains caused Nikolsky sign an hour or two after subcutaneous application of sterile concentrated supernatant of the strains investigated. On the basis of the investigation carried out it was concludes that for the occurrence of typical signs of EEP in biological experiment, besides the ability of producing the exfoliative toxin, some other favorable factors, not yet completely known, are also necessary.

Izvorni jezik
Hrvatski

Znanstvena područja
Veterinarska medicina

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