Naslov
Efficient shoot organogenesis of begonia (Begonia rex Putz.) induced by thidiazuron

Autori
Jelaska, Sibila ; Bauer, Nataša ; Kosor, Ela ; Leljak-Levanić, Dunja

Izvornik
Acta Botanica Croatica (0365-0588) 60 (2001), 2; 157-168

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Begonia rex; leaf disc; micropropagation; organogenesis; petiole segment; thidiazuron

Sažetak
The effects of thidiazuron (TDZ) on adventitious shoot bud formation from leaf and petiole explants excised from in-vitro-grown plantlets of a begonia were tested by a two-step procedure: a short exposure (5 to 120 min) to 40 µM TDZ in liquid MS medium followed by culture on agar-solidified MS medium without growth regulators. Exposure of explants for five min to TDZ induced bud formation, but the number of buds was increased with prolonged exposure. Sixty min exposure to TDZ resulted in the highest mean number of regenerated shoots (24/leaf disc; 44/petiole segment after five weeks of culture). Shoots generated after two week in culture without, or with a small amount of, callus. During the third and fourth week in culture shoots developed small roots, and from the mother explant separated shoots elongated and further developed. The sucrose concentration in the basal expression medium influenced the efficiency of shoot organogenesis. The frequency of shoot formation tended to increase when the medium was supplemented with 2% sucrose instead with 3%.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA