Naslov
Separation and identification of chlorogenic acids using the HPLC method

Autori
Tomac, Ivana ; Jakobek, Lidija ; Šeruga, Marijan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka 10. međunarodnog znanstveno- stručnog skupa Hranom do zdravlja / Šubarić, Drago ; Jašić, Midhat - Osijek : Tuzla : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku ; Farmaceutski fakultet Univerziteta u Tuzli, 2017, 99-99

ISBN
978-953-7005-49-8

Skup
10. međunarodni znanstveno-stručni skup ''Hranom do zdravlja''

Mjesto i datum
Osijek, Hrvatska, 12.10.2017. - 13.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chlorogenic acids, HPLC, validation

Sažetak
Chlorogenic acids (CGAs) are a group of polyphenolic compounds distributed in different plants. CGAs are a family of different esters formed between the (-)-quinic acid and certain trans-cinnamic acids (caffeic, ferulic). In this study, the separation and identification of nine different CGAs isomers: 5-O- caffeoylquinic acid (5-CQA), 4-O-caffeoylquinic acid (4-CQA), 3-O-caffeoylquinic acid (3-CQA), 3, 5-O-dicaffeoylquinic acid (3, 5-diCQA), 4, 5-O- dicaffeoylquinic acid (4, 5-diCQA), 3, 4- O- dicaffeoylquinic acid (3, 4-diCQA) and 5-O- feruloylquinic acid (5-FQA), 4-O-feruloylquinic acid (4-FQA), and 3-O-feruloylquinic acid (3- FQA) was performed in a model solution using high performance liquid chromatography (HPLC). The HPLC analysis was carried out on the HPLC system (Agilent) with a PDA detector using the C18 column and two mobile phases, 0.1% phosphoric acid in water, and 100% methanol. UV/Vis spectra were recorded in the wavelength range from 200 nm to 600 nm. Chlorogenic acids have shown the maximum absorbance at the wavelength of 320 nm. The order of eluation of the individual chlorogenic acids is consistent with the literature data. All investigated chlorogenic acids are well separated, except the 3, 4 – and 3, 5-diCQA (the retention times are very close). The method has shown linearity in the range of concentrations from 20 mg/L to 300 mg/L, limit of detection (LOD) was 1.7 · 10-5mol/L and the limit of quantification (LOQ) was 5.7 · 10-5mol/L. This method could be applied in the analysis of chlorogenic acids in various beverages.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA