Naslov
Basic Haematology and Cytochemical Characteristics of Blood Cells from two Freshwater Turtle Species: Mauremys rivulata (Valenciennes in Bory de Saint-Vincent 1833) and Emys orbicularis (Linnaeus 1758) in Croatia

Autori
Perutka, Jan ; Karaica, Dean ; Hodić, Sandra ; Cedilak, Matea ; Štih, Ana ; Čavčić, Ana ; Krizmanić, Aleksandra ; Đikić, Domagoj

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts Cutting Edge 2017: Scientific conference for young researchers / Kljun, Jakob ; Gaber, Aljaž - Ljubljana : Jupiter mediji d.o.o., 2017, 46-47

ISBN
978-961-6756-83-9

Skup
Cutting Edge 2017: SCIENTIFIC CONFERENCE FOR YOUNG RESEARCHERS

Mjesto i datum
Ljubljana, Slovenija, 19.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Haematology ; cytology ; endangered species

Sažetak
The Balkan Terrapin (Mauremys rivulata) is an endangered species that inhabits the area from Dalmatia, Albania, Greece and Cyprus all the way to Bulgaria1. In the Republic of Croatia, it is protected by law since only three populations remain in the Dubrovnik-Neretva County. The biggest population is located in Konavosko polje and is threatened by the degradation and disappearance of habitats (e.g. concreting of the channels and rivers). M. rivulata shares its habitat with the population of European Pond Turtle (Emys orbicularis). Using haematological and differential cytochemical techniques on the blood samples from 34 M. rivulata and 21 E. orbicularis individuals, their erythrocyte and leukocyte cells were characterised. Additional six healthy adult captive M. rivulata from the Zagreb ZOO were also sampled for determination of reliable erythrocyte and leukocyte reference counts. The aim of this study was to assess physiologicalcondition of this M. rivulata population. Polysaccharides and glycoconjugates were detected using the AB(Alcian Blue) and PAS (Periodic acid-Schiff) stains while ALP (Alkaline Phosphatase Enzyme) was detected using the appropriate enzymecytochemical method. M. rivulata leukocyte(5940/mm3)and erythrocyte(510000/mm3) count mean values were slightly greater than the literature mean values (leukocyte = 3500-5000/mm3 ; erythrocyte = 405333/mm3)2. The AB stained the nuclei of all cells ; eosinophils were detected in both whereas monocytes only in the blood of M. rivulata. Using PAS and ALP staining, lymphocytes and eosinophils were identified in both species. PAS stained heterophils of M. rivulata and thrombocytes of E. orbicularis whereas ALP stained heterophils and thrombocytes of M. rivulata and monocytes of E. orbicularis. This study is the first report of basic haematology and blood cell cytochemical data from two Testudines species inhabiting freshwater habitats in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA